Neonatal hypothyroidism permanently alters follicle-stimulating hormone and luteinizing hormone production in the male rat

John D. Kirby*, Niromi Arambepola, Tarja Porkka-Heiskanen, Yvonne K. Kirby, Marsha L. Rhoads, Hiroaki Nitta, Amy E. Jetton, Gary Iwamoto, Gary L. Jackson, Fred W. Turek, Paul S. Cooke

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Transient neonatal hypothyroidism, induced with the goitrogen 6-n- propyl-2-thiouracil (PTU), results in dramatic increases in both testis size and sperm production in the adult rat. The observed increases in testis size and function occur in the presence of normal circulating testosterone levels. However, circulating gonadotropin levels are chronically reduced by 30-50% at all times in treated males. To better understand the permanent reduction in serum gonadotropin levels following transient neonatal hypothyroidism, we conducted a series of experiments to evaluate pituitary and hypothalamic function in the adult male PTU-treated rat. PTU treatment led to a significant reduction in GnRH-stimulated LH production. Castration resulted in 3.9- to 8.5-fold increases in circulating gonadotropin levels in both treated and control males; however, the absolute increases were significantly reduced in treated males. In contrast to circulating levels, pituitary gonadotropin contents did not increase in treated males after castration. PTU treatment did not lead to a reduction in the density of either luteotropes or folliculotropes, and both cell types increased in size and density after castration. The relative concentrations of both gonadotropin β-subunit messenger RNAs increased more slowly in treated males than in controls after castration. Thus, although treated rats have the intrinsic ability to produce normal circulating levels of LH and FSH, gonadal feedback and an overall reduction in gonadotrope synthetic ability combine to produce the chronically reduced circulating levels of these hormones.

Original languageEnglish (US)
Pages (from-to)2713-2721
Number of pages9
Issue number7
StatePublished - 1997

ASJC Scopus subject areas

  • Endocrinology


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