Neoplastic conversion of human urothelial cells in vitro by overexpression of H2O2-generating peroxisomal fatty acyl CoA oxidase.

T. Tamatani*, K. Hattori, K. Nakashiro, Y. Hayashi, S. Wu, D. Klumpp, J. K. Reddy, R. Oyasu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

An in vitro study was conducted to determine if malignant transformation can be induced in human urothelial cells immortalized with human papillomavirus E6/E7 genes. A clone designated 1T1 was isolated and then stably transfected with an acyl CoA oxidase (ACOX)-expression construct. The cells generated H2O2 in a large quantity from the substrate linoleic acid (LA). After 56 days of LA treatment, cells persistently formed an epithelial cyst in athymic nude mice with an occasional intracystic epithelial nodule. Our results indicate that human urothelial cells can be transformed to low grade neoplastic cells by H2O2 and suggest that H2O2 may be involved in the development of bladder cancer.

Original languageEnglish (US)
Pages (from-to)743-749
Number of pages7
JournalInternational journal of oncology
Volume15
Issue number4
DOIs
StatePublished - Oct 1999

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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