Neoplastic conversion of human urothelial cells in vitro by overexpression of H2O2-generating peroxisomal fatty acyl CoA oxidase.

T. Tamatani; K. Hattori; K. Nakashiro; Y. Hayashi; S. Wu; D. Klumpp; J. K. Reddy; R. Oyasu

doi:10.3892/ijo.15.4.743

Neoplastic conversion of human urothelial cells in vitro by overexpression of H2O2-generating peroxisomal fatty acyl CoA oxidase.

T. Tamatani^*, K. Hattori, K. Nakashiro, Y. Hayashi, S. Wu, D. Klumpp, J. K. Reddy, R. Oyasu

^*Corresponding author for this work

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Abstract

An in vitro study was conducted to determine if malignant transformation can be induced in human urothelial cells immortalized with human papillomavirus E6/E7 genes. A clone designated 1T1 was isolated and then stably transfected with an acyl CoA oxidase (ACOX)-expression construct. The cells generated H2O2 in a large quantity from the substrate linoleic acid (LA). After 56 days of LA treatment, cells persistently formed an epithelial cyst in athymic nude mice with an occasional intracystic epithelial nodule. Our results indicate that human urothelial cells can be transformed to low grade neoplastic cells by H2O2 and suggest that H2O2 may be involved in the development of bladder cancer.

Original language	English (US)
Pages (from-to)	743-749
Number of pages	7
Journal	International journal of oncology
Volume	15
Issue number	4
DOIs	https://doi.org/10.3892/ijo.15.4.743
State	Published - Oct 1999

ASJC Scopus subject areas

Oncology
Cancer Research

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Neoplastic conversion of human urothelial cells in vitro by overexpression of H2O2-generating peroxisomal fatty acyl CoA oxidase.",

abstract = "An in vitro study was conducted to determine if malignant transformation can be induced in human urothelial cells immortalized with human papillomavirus E6/E7 genes. A clone designated 1T1 was isolated and then stably transfected with an acyl CoA oxidase (ACOX)-expression construct. The cells generated H2O2 in a large quantity from the substrate linoleic acid (LA). After 56 days of LA treatment, cells persistently formed an epithelial cyst in athymic nude mice with an occasional intracystic epithelial nodule. Our results indicate that human urothelial cells can be transformed to low grade neoplastic cells by H2O2 and suggest that H2O2 may be involved in the development of bladder cancer.",

author = "T. Tamatani and K. Hattori and K. Nakashiro and Y. Hayashi and S. Wu and D. Klumpp and Reddy, {J. K.} and R. Oyasu",

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AU - Tamatani, T.

AU - Hattori, K.

AU - Nakashiro, K.

AU - Hayashi, Y.

AU - Wu, S.

AU - Klumpp, D.

AU - Reddy, J. K.

AU - Oyasu, R.

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N2 - An in vitro study was conducted to determine if malignant transformation can be induced in human urothelial cells immortalized with human papillomavirus E6/E7 genes. A clone designated 1T1 was isolated and then stably transfected with an acyl CoA oxidase (ACOX)-expression construct. The cells generated H2O2 in a large quantity from the substrate linoleic acid (LA). After 56 days of LA treatment, cells persistently formed an epithelial cyst in athymic nude mice with an occasional intracystic epithelial nodule. Our results indicate that human urothelial cells can be transformed to low grade neoplastic cells by H2O2 and suggest that H2O2 may be involved in the development of bladder cancer.

AB - An in vitro study was conducted to determine if malignant transformation can be induced in human urothelial cells immortalized with human papillomavirus E6/E7 genes. A clone designated 1T1 was isolated and then stably transfected with an acyl CoA oxidase (ACOX)-expression construct. The cells generated H2O2 in a large quantity from the substrate linoleic acid (LA). After 56 days of LA treatment, cells persistently formed an epithelial cyst in athymic nude mice with an occasional intracystic epithelial nodule. Our results indicate that human urothelial cells can be transformed to low grade neoplastic cells by H2O2 and suggest that H2O2 may be involved in the development of bladder cancer.

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Neoplastic conversion of human urothelial cells in vitro by overexpression of H2O2-generating peroxisomal fatty acyl CoA oxidase.

Abstract

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