TY - JOUR
T1 - Nesprin-2 interacts with α-catenin and regulates Wnt signaling at the nuclear envelope
AU - Neumann, Sascha
AU - Schneider, Maria
AU - Daugherty, Rebecca L.
AU - Gottardi, Cara J.
AU - Eming, Sabine A.
AU - Beijer, Asa
AU - Noegel, Angelika A.
AU - Karakesisoglou, Iakowos
PY - 2010/11/5
Y1 - 2010/11/5
N2 - Nesprins and emerin are structural nuclear envelope proteins that tether nuclei to the cytoskeleton. In this work, we identified the cytoskeleton- associated α-N/E-catenins as novel nesprin-2-binding partners. The association involves the C termini of nesprin-2 giant and α-N/E-catenins. α-E/T/N-catenins are known primarily for their roles in cadherin-mediated cell-cell adhesion. Here, we show that, in addition, α-catenin forms complexes with nesprin-2 that include β-catenin and emerin. We demonstrate that the depletion of nesprin-2 reduces both the amount of active β-catenin inside the nucleus and T-cell factor/lymphoid-enhancing factor-dependent transcription. Taken together, these findings suggest novel nesprin-2 functions in cellular signaling. Moreover, we propose that, in contrast to emerin, nesprin-2 is a positive regulator of the Wnt signaling pathway.
AB - Nesprins and emerin are structural nuclear envelope proteins that tether nuclei to the cytoskeleton. In this work, we identified the cytoskeleton- associated α-N/E-catenins as novel nesprin-2-binding partners. The association involves the C termini of nesprin-2 giant and α-N/E-catenins. α-E/T/N-catenins are known primarily for their roles in cadherin-mediated cell-cell adhesion. Here, we show that, in addition, α-catenin forms complexes with nesprin-2 that include β-catenin and emerin. We demonstrate that the depletion of nesprin-2 reduces both the amount of active β-catenin inside the nucleus and T-cell factor/lymphoid-enhancing factor-dependent transcription. Taken together, these findings suggest novel nesprin-2 functions in cellular signaling. Moreover, we propose that, in contrast to emerin, nesprin-2 is a positive regulator of the Wnt signaling pathway.
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U2 - 10.1074/jbc.M110.119651
DO - 10.1074/jbc.M110.119651
M3 - Article
C2 - 20801886
AN - SCOPUS:78049402790
SN - 0021-9258
VL - 285
SP - 34932
EP - 34938
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 45
ER -