Nested chromosomal deletions induced with retroviral vectors in mice

Hong Su, Xiaozhong Wang, Allan Bradley*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


Chromosomal deletions, especially nested deletions, are major genetic tools in diploid organisms that facilitate the functional analysis of large chromosomal regions and allow the rapid localization of mutations to specific genetic intervals. In mice, well-characterized overlapping deletions are only available at a few chromosomal loci, partly due to drawbacks of existing methods. Here we exploit the random integration of a retrovirus to generate high-resolution sets of nested deletions around defined loci in embryonic stem (ES) cells, with sizes extending from a few kilobases to several megabases. This approach expands the application of Cre-loxP-based chromosome engineering because it not only allows the construction of hundreds of overlapping deletions, but also provides molecular entry points to regions based on the retroviral tags. Our approach can be extended to any region of the mouse genome.

Original languageEnglish (US)
Pages (from-to)92-95
Number of pages4
JournalNature Genetics
Issue number1
StatePublished - 2000

ASJC Scopus subject areas

  • Genetics


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