Neurite extension in central neurons: A novel role for the receptor tyrosine kinases Ror1 and Ror2

Sabrina Paganoni, Adriana B Ferreira*

*Corresponding author for this work

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Neurite elongation and branching are key cellular events during brain development as they underlie the formation of a properly wired neuronal network. Here we report that the receptor tyrosine kinases Ror1 and Ror2 modulate the growth of neurites as well as their branching pattern in hippocampal neurons. Upon Ror1 or Ror2 suppression using antisense oligonucleotides or RNA interference (RNAi), neurons extended shorter and less branched minor processes when compared to those in control cells. In addition, Ror-depleted cells elongated longer, albeit less branched, axons than seen in control cells. Conversely, Ror overexpression both in non-neuronal cells and in hippocampal neurons resulted in the enhanced extension of short and highly branched processes. These phenotypes were accompanied by changes in the microtubule-associated proteins MAP1B and MAP2. Taken together, these results support a novel role for Ror receptors as modulators of neurite extension in central neurons.

Original languageEnglish (US)
Pages (from-to)433-446
Number of pages14
JournalJournal of Cell Science
Volume118
Issue number2
DOIs
StatePublished - Jan 15 2005

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Keywords

  • Antisense
  • Microtubule-associated proteins
  • Neurite elongation and branching
  • Receptor tyrosine kinases
  • siRNA

ASJC Scopus subject areas

  • Cell Biology

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