Neurochemical Characteristics of a Novel Dorsal Root Ganglion X Neuroblastoma Hybrid Cell Line, F‐11

Paul C. Francel, Kathy Harris, Michelle Smith, Mark C. Fishman, Glyn Dawson*, Richard J. Miller

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

Abstract: We investigated the properties of the novel dorsal root ganglion (DRG) hybrid cell line F‐11 to see how closely these cells resembled normal DRG cells. Under normal growth conditions, F‐11 cells appeared to contain several short neurite‐like processes. However, these cells could also be grown under conditions in which they showed a much more extensive neuronal morphology, exhibiting many long neurites. Several differentiated features of DRG cells were present on F‐11 cells. These included the presence of δ5‐opioid receptors, receptors for prostaglandins and brady‐kinin, and dihydropyridine‐sensitive calcium channels. F‐11 cells also synthesized and released a substance P‐Iike compound, as determined by immunoreactivity. Both the number of bradykinin receptors and the voltage‐sensitive calcium influx increased on cell differentiation. Opioid agonists (δ‐specificity) were found to decrease cyclic AMP levels in F‐l 1 cells in a naloxone‐ and pertussis toxin‐reversible fashion. Bradykinin stimulated the synthesis of inositol‐1,4‐bisphosphate and inositol‐1,4,5‐trisphosphate. Ca2+ channel agonists stimulated voltage‐sensitive Ca2+ influx in a dose‐dependent, stereospecific manner, whereas Ca2+ channel antagonists inhibited Ca2+ influx. F‐ll cells should, therefore, prove useful as models for authentic DRG neurons.

Original languageEnglish (US)
Pages (from-to)1624-1631
Number of pages8
JournalJournal of neurochemistry
Volume48
Issue number5
DOIs
StatePublished - May 1987

Keywords

  • Bradykinin
  • Calcium channels
  • Dorsal root ganglion
  • Opiate receptors
  • Prostanoids
  • Substance P‐like immunoreactivity

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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