Neurohumoral control of ileal electrolyte transport. I. Bombesin and related peptides

J. F. Kachur, R. J. Miller, M. Field, J. Rivier

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


The tetradecapeptide bombesin caused a transient increase in transepithelial potential difference and short-circuit current (lsc) when added to the medium bathing the serosal surface of guinea-pig or rat ileal mucosa mounted in vitro in Ussing chambers. Bombesin added to the mucosal bathing medium was ineffective. Bombesin caused a maximal increase of 66 μA/cm2 in lsc. A half-maximal increase was obtained with 4.5 x 10-9 M bombesin. Ranatensin and gastrin-releasing peptide, two naturally occurring peptides with similar sequences to bombesin, also produced large increases in lsc. After addition of bombesin, subsequent additions of peptide exhibited desensitization. Cross-desensitization occurred between bombesin and other similar peptides such as ranatensin, but not with the peptides substance P and neurotensin or with serotonin which also stimulated lsc. Bombesin-induced desensitization was reversible. The effect of bombesin was not blocked by atropine, indomethacin, diphenhydramine, etorphine, somatostatin, epinephrine, tetrodotoxin or verapamil. Replacement of Cl and HCO3 with gluconate or SO4 in the Ringer's solution only slightly reduced the effect of bombesin in the guinea pig, but greatly reduced it in the rat. Replacement of Na with choline reduced the action of bombesin substantially in the guinea pig. A combination of a maximally effective concentration of bombesin with prostaglandin E1 and theophylline only produced a total increment in lsc equal to that produced by bombesin alone. The results suggest that bombesin stimulates active anion secretion by acting on a specific bombesin receptor localized on the serosal surface of ileal epithelial cells.

Original languageEnglish (US)
Pages (from-to)449-455
Number of pages7
JournalJournal of Pharmacology and Experimental Therapeutics
Issue number3
StatePublished - 1982

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology


Dive into the research topics of 'Neurohumoral control of ileal electrolyte transport. I. Bombesin and related peptides'. Together they form a unique fingerprint.

Cite this