Neuromodulation of excitatory synaptogenesis in striatal development

Yevgenia Kozorovitskiy; Rui Peixoto; Wengang Wang; Arpiar Saunders; Bernardo L. Sabatini

doi:10.7554/eLife.10111.001

Neuromodulation of excitatory synaptogenesis in striatal development

Yevgenia Kozorovitskiy, Rui Peixoto, Wengang Wang, Arpiar Saunders, Bernardo L. Sabatini^*

^*Corresponding author for this work

Neurobiology

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Dopamine is released in the striatum during development and impacts the activity of Protein Kinase A (PKA) in striatal spiny projection neurons (SPNs). We examined whether dopaminergic neuromodulation regulates activity-dependent glutamatergic synapse formation in the developing striatum. Systemic in vivo treatment with Gα_s-coupled G-protein receptors (GPCRs) agonists enhanced excitatory synapses on direct pathway striatal spiny projection neurons (dSPNs), whereas rapid production of excitatory synapses on indirect pathway neurons (iSPNs) required the activation of Gα_s GPCRs in SPNs of both pathways. Nevertheless, in vitro Gα_s activation was sufficient to enhance spinogenesis induced by glutamate photolysis in both dSPNs and iSPNs, suggesting that iSPNs in intact neural circuits have additional requirements for rapid synaptic development. We evaluated the in vivo effects of enhanced glutamate release from corticostriatal axons and postsynaptic PKA and discovered a mechanism of developmental plasticity wherein rapid synaptogenesis is promoted by the coordinated actions of glutamate and postsynaptic Gα_s-coupled receptors.

Original language	English (US)
Article number	e10111
Journal	eLife
Volume	4
Issue number	NOVEMBER2015
DOIs	https://doi.org/10.7554/eLife.10111.001
State	Published - Nov 9 2015

Funding

The authors thank Rachel Pemberton for genotyping, Matt Banghart and Ruchir Shah for help with the SEAP assay, Lai Ding (Harvard NeuroDiscovery Center) for ImageJ macros, and all members of the Sabatini laboratory for helpful discussions. YK was supported by Leonard and Isabelle Goldenson Research Fellowship, the Nancy Lurie Marks Family Foundation, and William N. and Bernice E. Bumpus Foundation Innovation Award. RP was supported by the Alice and Joseph Brooks fellowship and the Nancy Lurie Marks Family Foundation. This work was supported by NS046579 and Howard Hughes Medical Institute (BLS). The authors thank Rachel Pemberton for genotyping, Matt Banghart and Ruchir Shah for help with the SEAP assay, Lai Ding (Harvard NeuroDiscovery Center) for ImageJ macros, and all members of the Sabatini laboratory for helpful discussions. YK was supported by Leonard and Isabelle Goldenson Research Fellowship, the Nancy Lurie Marks Family Foundation, and William N. and Bernice E. Bum-pus Foundation Innovation Award. RP was supported by the Alice and Joseph Brooks fellowship and the Nancy Lurie Marks Family Foundation. This work was supported by NS046579 and Howard Hughes Medical Institute (BLS).

ASJC Scopus subject areas

General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology
General Neuroscience

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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abstract = "Dopamine is released in the striatum during development and impacts the activity of Protein Kinase A (PKA) in striatal spiny projection neurons (SPNs). We examined whether dopaminergic neuromodulation regulates activity-dependent glutamatergic synapse formation in the developing striatum. Systemic in vivo treatment with Gαs-coupled G-protein receptors (GPCRs) agonists enhanced excitatory synapses on direct pathway striatal spiny projection neurons (dSPNs), whereas rapid production of excitatory synapses on indirect pathway neurons (iSPNs) required the activation of Gαs GPCRs in SPNs of both pathways. Nevertheless, in vitro Gαs activation was sufficient to enhance spinogenesis induced by glutamate photolysis in both dSPNs and iSPNs, suggesting that iSPNs in intact neural circuits have additional requirements for rapid synaptic development. We evaluated the in vivo effects of enhanced glutamate release from corticostriatal axons and postsynaptic PKA and discovered a mechanism of developmental plasticity wherein rapid synaptogenesis is promoted by the coordinated actions of glutamate and postsynaptic Gαs-coupled receptors.",

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Neuromodulation of excitatory synaptogenesis in striatal development

Abstract

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ASJC Scopus subject areas

UN SDGs

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