Neuronal progenitor transplantation and respiratory outcomes following upper cervical spinal cord injury in adult rats

Todd E. White; Michael A. Lane; Milapjit S. Sandhu; Barbara E. O'Steen; David D. Fuller; Paul J. Reier

doi:10.1016/j.expneurol.2010.06.006

Neuronal progenitor transplantation and respiratory outcomes following upper cervical spinal cord injury in adult rats

Todd E. White, Michael A. Lane, Milapjit S. Sandhu, Barbara E. O'Steen, David D. Fuller, Paul J. Reier^*

^*Corresponding author for this work

Physical Medicine and Rehabilitation

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Despite extensive gray matter loss following spinal cord injury (SCI), little attention has been given to neuronal replacement strategies and their effects on specific functional circuits in the injured spinal cord. In the present study, we assessed breathing behavior and phrenic nerve electrophysiological activity following transplantation of microdissected dorsal or ventral pieces of rat fetal spinal cord tissue (FSC_D or FSC_V, respectively) into acute, cervical (C2) spinal hemisections. Transneuronal tracing demonstrated connectivity between donor neurons from both sources and the host phrenic circuitry. Phrenic nerve recordings revealed differential effects of dorsally vs. ventrally derived neural progenitors on ipsilateral phrenic nerve recovery and activity. These initial results suggest that local gray matter repair can influence motoneuron function in targeted circuits following spinal cord injury and that outcomes will be dependent on the properties and phenotypic fates of the donor cells employed.

Original language	English (US)
Pages (from-to)	231-236
Number of pages	6
Journal	Experimental Neurology
Volume	225
Issue number	1
DOIs	https://doi.org/10.1016/j.expneurol.2010.06.006
State	Published - Sep 2010

Funding

Support for this work was provided by NIH NINDS RO1NS054025 (P.J.R.) and the Anne and Oscar Lackner Chair in Medicine (P.J.R.). The authors also wish to express their appreciation to Mr. John Meyer for his outstanding technical assistance and to Dr. L.W. Enquist (Princeton Univ.) for providing PRV 152 as a service of the National Center for Experimental Neuroanatomy with Neurotropic Viruses (NCRR P40 RR01 18604).

Keywords

Cervical spinal cord injury
Fetal spinal cord
Phrenic
Rats
Respiratory
Transplantation
Ventilation

ASJC Scopus subject areas

Neurology
Developmental Neuroscience

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10.1016/j.expneurol.2010.06.006

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abstract = "Despite extensive gray matter loss following spinal cord injury (SCI), little attention has been given to neuronal replacement strategies and their effects on specific functional circuits in the injured spinal cord. In the present study, we assessed breathing behavior and phrenic nerve electrophysiological activity following transplantation of microdissected dorsal or ventral pieces of rat fetal spinal cord tissue (FSCD or FSCV, respectively) into acute, cervical (C2) spinal hemisections. Transneuronal tracing demonstrated connectivity between donor neurons from both sources and the host phrenic circuitry. Phrenic nerve recordings revealed differential effects of dorsally vs. ventrally derived neural progenitors on ipsilateral phrenic nerve recovery and activity. These initial results suggest that local gray matter repair can influence motoneuron function in targeted circuits following spinal cord injury and that outcomes will be dependent on the properties and phenotypic fates of the donor cells employed.",

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Neuronal progenitor transplantation and respiratory outcomes following upper cervical spinal cord injury in adult rats

Abstract

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Keywords

ASJC Scopus subject areas

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