Neuronal Puncta/Aggregate Formation by WT and Mutant UBQLN2

Nathaniel Safren, Lisa M. Sharkey, Sami J. Barmada*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Protein aggregates are a common feature of nearly all neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis (ALS). Here we describe a method to quickly and accurately measure protein aggregation in cells expressing a fluorescently tagged aggregation-prone protein. This unbiased method obviates the need for manual scoring and facilitates the identification of factors governing protein self-assembly and its downstream consequences for cell heath.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages561-573
Number of pages13
DOIs
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2551
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Longitudinal microscopy
  • Neurodegeneration
  • Protein aggregation
  • Proteostasis
  • Single cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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