Neuropathology of degenerative cell death in Caenorhabditis elegans

David H. Hall, Guoqiang Gu, Jaime García-Añoveros, Lei Gong, Martin Chalfie*, Monica Driscoll

*Corresponding author for this work

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

In Caenorhabditis elegans necrosis-like neuronal death is induced by gain-of-function (gf) mutations in two genes, mec-4 and dog-1, that encode proteins similar to subunits of the vertebrate amiloride-sensitive epithelial Na+ channel. We have determined the progress of cellular pathology in dying neurons via light and electron microscopy. The first detectable abnormality is an infolding of the plasma membrane and the production of small electron- dense whorls. Later, cytoplasmic vacuoles and larger membranous whorls form, and the cell swells. More slowly, chromatin aggregates and the nucleus invaginates. Mitochondria and Golgi are not dramatically affected until the final stages of cell death when organelles, and sometimes the cells themselves, lyse. Certain cells, including some muscle cells in dog-1 animals, express the abnormal gene products and display a few membrane abnormalities but do not die. These cells either express the mutant genes at lower levels, lack other proteins needed to form inappropriately functioning channels, or are better able to compensate for the toxic effects of the channels. Overall, the ultrastructural changes in these deaths suggest that enhanced membrane cycling precludes vacuolation and cell swelling. The pathology of mec-4(gf) and deg-1(gf) cells shares features with that of genetic disorders with alterations in channel subunits, such as hypokalemic periodic paralysis in humans and the weaver mouse, and with degenerative conditions, e.g., acute excitotoxic death. The initial pathology in all of these conditions may reflect attempts by affected cells to compensate for abnormal membrane proteins or functions.

Original languageEnglish (US)
Pages (from-to)1033-1045
Number of pages13
JournalJournal of Neuroscience
Volume17
Issue number3
StatePublished - Feb 1 1997

Fingerprint

Caenorhabditis elegans
Cell Death
Pathology
Hypokalemic Periodic Paralysis
Dogs
Neurologic Mutant Mice
Genes
Epithelial Sodium Channels
Inborn Genetic Diseases
Membranes
Amiloride
Poisons
Vacuoles
Neuropathology
Organelles
Muscle Cells
Chromatin
Vertebrates
Electron Microscopy
Mitochondria

Keywords

  • Caenorhabditis elegans
  • deg-1 neuropathology
  • degenerin
  • mec-4
  • membrane cycling
  • necrosis
  • neurodegeneration

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hall, D. H., Gu, G., García-Añoveros, J., Gong, L., Chalfie, M., & Driscoll, M. (1997). Neuropathology of degenerative cell death in Caenorhabditis elegans. Journal of Neuroscience, 17(3), 1033-1045.
Hall, David H. ; Gu, Guoqiang ; García-Añoveros, Jaime ; Gong, Lei ; Chalfie, Martin ; Driscoll, Monica. / Neuropathology of degenerative cell death in Caenorhabditis elegans. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 3. pp. 1033-1045.
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Hall, DH, Gu, G, García-Añoveros, J, Gong, L, Chalfie, M & Driscoll, M 1997, 'Neuropathology of degenerative cell death in Caenorhabditis elegans', Journal of Neuroscience, vol. 17, no. 3, pp. 1033-1045.

Neuropathology of degenerative cell death in Caenorhabditis elegans. / Hall, David H.; Gu, Guoqiang; García-Añoveros, Jaime; Gong, Lei; Chalfie, Martin; Driscoll, Monica.

In: Journal of Neuroscience, Vol. 17, No. 3, 01.02.1997, p. 1033-1045.

Research output: Contribution to journalArticle

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T1 - Neuropathology of degenerative cell death in Caenorhabditis elegans

AU - Hall, David H.

AU - Gu, Guoqiang

AU - García-Añoveros, Jaime

AU - Gong, Lei

AU - Chalfie, Martin

AU - Driscoll, Monica

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N2 - In Caenorhabditis elegans necrosis-like neuronal death is induced by gain-of-function (gf) mutations in two genes, mec-4 and dog-1, that encode proteins similar to subunits of the vertebrate amiloride-sensitive epithelial Na+ channel. We have determined the progress of cellular pathology in dying neurons via light and electron microscopy. The first detectable abnormality is an infolding of the plasma membrane and the production of small electron- dense whorls. Later, cytoplasmic vacuoles and larger membranous whorls form, and the cell swells. More slowly, chromatin aggregates and the nucleus invaginates. Mitochondria and Golgi are not dramatically affected until the final stages of cell death when organelles, and sometimes the cells themselves, lyse. Certain cells, including some muscle cells in dog-1 animals, express the abnormal gene products and display a few membrane abnormalities but do not die. These cells either express the mutant genes at lower levels, lack other proteins needed to form inappropriately functioning channels, or are better able to compensate for the toxic effects of the channels. Overall, the ultrastructural changes in these deaths suggest that enhanced membrane cycling precludes vacuolation and cell swelling. The pathology of mec-4(gf) and deg-1(gf) cells shares features with that of genetic disorders with alterations in channel subunits, such as hypokalemic periodic paralysis in humans and the weaver mouse, and with degenerative conditions, e.g., acute excitotoxic death. The initial pathology in all of these conditions may reflect attempts by affected cells to compensate for abnormal membrane proteins or functions.

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Hall DH, Gu G, García-Añoveros J, Gong L, Chalfie M, Driscoll M. Neuropathology of degenerative cell death in Caenorhabditis elegans. Journal of Neuroscience. 1997 Feb 1;17(3):1033-1045.