Neuropeptide degradation by large vessel and microvessel-derived endothelial cells in vitro: cell surface catabolism of thyrotropin releasing hormone (TRH)

Jack M. Rozental; Grazyna Kaminska; Joel Turner; Thomas Schwartz; Victoria Cadahia; Benjamin R. Brooks

doi:10.1016/0006-8993(89)90792-0

Neuropeptide degradation by large vessel and microvessel-derived endothelial cells in vitro: cell surface catabolism of thyrotropin releasing hormone (TRH)

Jack M. Rozental^*, Grazyna Kaminska, Joel Turner, Thomas Schwartz, Victoria Cadahia, Benjamin R. Brooks

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Cell surface ectopeptidase activity of purified, cultured large vessel and microvessel-derived endothelial cells (EC) was studied. Degradation of thyrotropin releasing hormone (TRH), and production of cyclo-His-Pro was significantly increased (P < 0.001) in large vessel EC compared with microcapillary EC. Since the rate of catabolism in the microvascular capillary bed is 5 times less than that in the large vessel wall, peptide concentrations are likely maintained longer in close proximity to their site of biosynthesis, where they are presumably most active.

Original language	English (US)
Pages (from-to)	397-401
Number of pages	5
Journal	Brain research
Volume	499
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(89)90792-0
State	Published - Oct 16 1989

Keywords

Aminopeptidase
Endothelial cell
Neuropeptide
Thyrotropin releasing hormone

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(89)90792-0

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title = "Neuropeptide degradation by large vessel and microvessel-derived endothelial cells in vitro: cell surface catabolism of thyrotropin releasing hormone (TRH)",

abstract = "Cell surface ectopeptidase activity of purified, cultured large vessel and microvessel-derived endothelial cells (EC) was studied. Degradation of thyrotropin releasing hormone (TRH), and production of cyclo-His-Pro was significantly increased (P < 0.001) in large vessel EC compared with microcapillary EC. Since the rate of catabolism in the microvascular capillary bed is 5 times less than that in the large vessel wall, peptide concentrations are likely maintained longer in close proximity to their site of biosynthesis, where they are presumably most active.",

keywords = "Aminopeptidase, Endothelial cell, Neuropeptide, Thyrotropin releasing hormone",

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Neuropeptide degradation by large vessel and microvessel-derived endothelial cells in vitro : cell surface catabolism of thyrotropin releasing hormone (TRH). / Rozental, Jack M.; Kaminska, Grazyna; Turner, Joel; Schwartz, Thomas; Cadahia, Victoria; Brooks, Benjamin R.

In: Brain research, Vol. 499, No. 2, 16.10.1989, p. 397-401.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Neuropeptide degradation by large vessel and microvessel-derived endothelial cells in vitro

T2 - cell surface catabolism of thyrotropin releasing hormone (TRH)

AU - Rozental, Jack M.

AU - Kaminska, Grazyna

AU - Turner, Joel

AU - Schwartz, Thomas

AU - Cadahia, Victoria

AU - Brooks, Benjamin R.

PY - 1989/10/16

Y1 - 1989/10/16

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AB - Cell surface ectopeptidase activity of purified, cultured large vessel and microvessel-derived endothelial cells (EC) was studied. Degradation of thyrotropin releasing hormone (TRH), and production of cyclo-His-Pro was significantly increased (P < 0.001) in large vessel EC compared with microcapillary EC. Since the rate of catabolism in the microvascular capillary bed is 5 times less than that in the large vessel wall, peptide concentrations are likely maintained longer in close proximity to their site of biosynthesis, where they are presumably most active.

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KW - Endothelial cell

KW - Neuropeptide

KW - Thyrotropin releasing hormone

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