Neuropeptide Y: a powerful modulator of epithelial ion transport

David D. Friel, Richard J. Miller*, Mary W. Walker

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


Neuropeptide Y (NPY) is a major gut peptide localized in the intestinal mucosa of several mammalian species. Ileal mucosa from rabbit and guinea‐pig was mounted in Ussing chambers in order to study the effect of NPY on short circuit current. Neuropeptide Y inhibited the short circuit current when applied to the serosal side of the tissue. The maximum change in short circuit current was − 50 ± 6 μA cm−2 in the rabbit ileum and − 49 ± 14 μA cm−2 in the guinea‐pig ileum. The EC50 was 3 × 10−8 M in both species. Pretreatment of rabbit ileum with the α2‐adrenoceptor antagonist, yohimbine (1 × 10−6 M) for 10 min did not reduce the response of the tissue to neuropeptide Y (1 × 10−7 M). When applied serosally to rabbit ileal mucosa, the related peptide YY caused a maximum change in short circuit current of − 60 ± 13 μA cm−2; the EC50 was 2 × 10−9 M. Isotopic flux studies in rabbit ileum showed that 1 × 10−7 M neuropeptide Y enhanced mucosal‐toserosal Na+ and Cl fluxes and reduced serosal‐to‐mucosal Cl flux. Replacement of chloride with gluconate on both sides of the tissue significantly reduced the change in short circuit current produced by neuropeptide Y (1 × 10−7 M), as did a similar replacement of bicarbonate. It is concluded that neuropeptide Y and peptide YY are the most potent neurotransmitters or hormones so far described in their ability to attenuate electrogenic transport in the small intestine. 1986 British Pharmacological Society

Original languageEnglish (US)
Pages (from-to)425-431
Number of pages7
JournalBritish journal of pharmacology
Issue number2
StatePublished - Jun 1986

ASJC Scopus subject areas

  • Pharmacology


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