Neuropeptide Y and pancreatic polypeptide reduce calcium currents in acutely dissociated neurons from adult rat superior cervical ganglia

S. Foucart, D. Bleakman, V. P. Bindokas, R. J. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

We examined the effects of neuropeptide Y (NPY) and pancreatic polypeptide on calcium currents (I(Ca)) in acutely dissociated neurons from the adult rat superior cervical ganglion. We found that NPY inhibited the I(Ca) with an estimated IC50 value of 140 nM. This inhibitory effect appeared to be restricted to a subset of cells which were smaller in diameter than the general population. The effect of NPY on the I(Ca) was prevented by pretreatment with pertussis toxin, suggesting the involvement of a GTP- binding protein of the G(i) or G(o) subtype. ω-conotoxin GVIA also occluded the effects of NPY, which suggests that these were directed toward N-type Ca++ channels. The effects of NPY were mimicked by the fragment NPY (13- 36) but not by peptide YY, indicating that a receptor distinct from a Y1- or a Y2-like NPY receptor was involved. Finally, we also observed that pancreatic polypeptide inhibited the I(Ca), suggesting that a pancreatic polypeptide receptor is also present on superior cervical ganglion neurons.

Original languageEnglish (US)
Pages (from-to)903-909
Number of pages7
JournalJournal of Pharmacology and Experimental Therapeutics
Volume265
Issue number2
StatePublished - 1993

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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