Abstract
The identity of the neuropeptide Y (NPY) receptor associated with the observed inhibition of neuronal Ca2+ currents (ICa) in rat dorsal root ganglion (DRG) cells has been established on the basis of agonist responses to analogues and carboxy terminal (C‐terminal) fragments of the NPY molecule. Whole cell barium currents (IBa) in DRG cells were reversibly inhibited by 100 nm NPY, 100 nm PYY and C‐terminal fragments of NPY in a manner that correlated with the length of the NPY fragments (for inhibition of the IBa NPY = PYY > NPY2–36 > NPY13–36 > NPY16–36 > NPY18–36 ≫ NPY25–36). C‐terminal fragments of NPY were also effective in reversibly reducing the ICa, the associated increase in the intracellular Ca2+ concentration ([Ca2+]i) and the increased [Ca2+]i produced by evoked action potentials in the DRG cells. In addition, a Ca2+‐activated Cl− conductance was also reversibly reduced by NPY fragments only when accompanied by a reduction in Ca2+ entry. We conclude that the Y2 receptor for neuropeptide Y is coupled to inhibition of Ca2+ influx via voltage‐sensitive calcium channels in DRG cells. 1991 British Pharmacological Society
Original language | English (US) |
---|---|
Pages (from-to) | 1781-1789 |
Number of pages | 9 |
Journal | British journal of pharmacology |
Volume | 103 |
Issue number | 3 |
DOIs | |
State | Published - Jul 1991 |
Keywords
- Y receptor
- calcium currents
- current clamp
- fura‐2
- intracellular calcium
- neuropeptide Y
- neuropeptide Y fragments
- sensory neurones
- voltage clamp
ASJC Scopus subject areas
- Pharmacology