Neuropeptide Y inhibits Ca2+ influx into cultured dorsal root ganglion neurones of the rat via a Y2 receptor

D. Bleakman*, W. F. Colmers, A. Fournier, R. J. Miller

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

The identity of the neuropeptide Y (NPY) receptor associated with the observed inhibition of neuronal Ca2+ currents (ICa) in rat dorsal root ganglion (DRG) cells has been established on the basis of agonist responses to analogues and carboxy terminal (C‐terminal) fragments of the NPY molecule. Whole cell barium currents (IBa) in DRG cells were reversibly inhibited by 100 nm NPY, 100 nm PYY and C‐terminal fragments of NPY in a manner that correlated with the length of the NPY fragments (for inhibition of the IBa NPY = PYY > NPY2–36 > NPY13–36 > NPY16–36 > NPY18–36 ≫ NPY25–36). C‐terminal fragments of NPY were also effective in reversibly reducing the ICa, the associated increase in the intracellular Ca2+ concentration ([Ca2+]i) and the increased [Ca2+]i produced by evoked action potentials in the DRG cells. In addition, a Ca2+‐activated Cl conductance was also reversibly reduced by NPY fragments only when accompanied by a reduction in Ca2+ entry. We conclude that the Y2 receptor for neuropeptide Y is coupled to inhibition of Ca2+ influx via voltage‐sensitive calcium channels in DRG cells. 1991 British Pharmacological Society

Original languageEnglish (US)
Pages (from-to)1781-1789
Number of pages9
JournalBritish journal of pharmacology
Volume103
Issue number3
DOIs
StatePublished - Jul 1991

Keywords

  • Y receptor
  • calcium currents
  • current clamp
  • fura‐2
  • intracellular calcium
  • neuropeptide Y
  • neuropeptide Y fragments
  • sensory neurones
  • voltage clamp

ASJC Scopus subject areas

  • Pharmacology

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