New mutations fts-36, lts-33, and ftsW clustered in the mra region of the Escherichia coli chromosome induce thermosensitive cell growth and division

F. Ishino, Kwan Jung Hai Kwan Jung, M. Ikeda, M. Doi, M. Wachi, M. Matsuhashi

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Three new mutants of Escherichia coli showing thermosensitive cell growth and division were isolated, and the mutations were mapped to the mra region at 2 min on the E. coli chromosome map distal to leuA. Two mutations were mapped closely upstream of ftsI (also called pbpB), in a region of 600 bases; the fts-36 mutant showed thermosensitive growth and formed filamentous cells at 42°C, whereas the lts-33 mutant lysed at 42°C without forming filamentous cells. The mutation in the third new thermosensitive, filament-forming mutant, named ftsW, was mapped between murF and murG. By isolation of these three mutants, about 90% of the 17-kilobase region from fts-36-lts-33 to envA could be filled with genes for cell division and growth, and the genes could be aligned.

Original languageEnglish (US)
Pages (from-to)5523-5530
Number of pages8
JournalJournal of bacteriology
Volume171
Issue number10
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'New mutations fts-36, lts-33, and ftsW clustered in the mra region of the Escherichia coli chromosome induce thermosensitive cell growth and division'. Together they form a unique fingerprint.

Cite this