Next-generation sequencing of the human olfactory receptors

Joel D. Mainland, Jason R. Willer, Hiroaki Matsunami, Elias Nicholas Katsanis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Humans have approximately 400 intact olfactory receptors (ORs). Among this set there are a large number of variations between individuals, a subset of which affects receptor function and can lead to interindividual variation in olfactory perception. Technological progress and cost erosion in next-generation sequencing have given us the opportunity to determine the sequence of the entire OR gene set with high fi delity and to measure the extent of variation in this functional module across many individuals. Given that whole genome sequencing remains prohibitively expensive for this purpose, especially since the OR sub-genome represents only ~0.0125 % of the human genome, we have designed a targeted capture method to enrich the OR for next-generation sequencing, which we describe here. Using this method we have been able to sequence an individual's OR sub-genome with high coverage, enabling us to identify variation with high sensitivity and speci fi city. This method can be used to accurate assess the amount of variability in this module and to identify the functional role of individual ORs in olfactory perception.

Original languageEnglish (US)
Title of host publicationProteomics for Biomarker Discovery
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages133-147
Number of pages15
ISBN (Print)9781627033763
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1003
ISSN (Print)1064-3745

Keywords

  • Illumina
  • Next-generation sequencing
  • Odor
  • Olfaction
  • Olfactory receptor
  • SNP
  • Targeted genomic enrichment

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Mainland, J. D., Willer, J. R., Matsunami, H., & Katsanis, E. N. (2013). Next-generation sequencing of the human olfactory receptors. In Proteomics for Biomarker Discovery: Methods and Protocols (pp. 133-147). (Methods in Molecular Biology; Vol. 1003). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-377-0_10