Nicotine induced murine spermatozoa apoptosis via up-regulation of deubiquitinated RIP1 by Trim27 promoter hypomethylation

Dongsheng Nie, Dong Zhang, Jingbo Dai, Meixing Zhang, Xianglong Zhao, Wangjie Xu, Zhong Chen, Lianyun Wang, Zhaoxia Wang, Zhongdong Qiao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Nicotine significantly promoted apoptosis in stages I, VII, VIII, and XI spermatogonia, stages I, VII, VIII, X, and XI spermatocytes, and stages I-V, VII, and VIII elongating spermatids. To explore the underlying molecular mechanisms, sperm mRNA next-generation sequencing of nicotine-treated mice was conducted. Out of the 86 genes related to apoptosis, Tnf (tumor necrosis factor alpha) was screened to be the most significant varied transcript, and the Onto-pathway analysis indicated that the TNF apoptotic pathway was especially activated by nicotine exposure. The TNF pathway was further studied at the gene and protein levels. The results showed that RIP1, the key component in the TNF apoptotic pathway, was up-expressed in its deubiquitinated form in nicotine-treated mice testis. TRIM27, an E3 ubiquitin ligase that activated TNF apoptotic pathway through up-regulating deubiquitinated RIP1, was also overexpressed in nicotine-treated spermatocytes; moreover, four consecutive CpG sites near the Trim27 transcription start site were less frequently methylated. Finally, in vitro experiments of Trim27 overexpression and RNA interference in GC-1 spermatogonial cells confirmed that the RIP1 deubiquitination and TRIM27 hyopmethylation were both positively correlated with spermatocyte apoptosis. In summary, our study suggests that nicotine may induce murine spermatozoal apoptosis via the TNF apoptotic pathway through up-regulation of deubiquitinated RIP1 by Trim27 promoter hypomethylation.

Original languageEnglish (US)
Article number31
JournalBiology of reproduction
Volume94
Issue number2
DOIs
StatePublished - Feb 2016

Keywords

  • Apoptosis
  • Nicotine
  • RIP1 deubiquitination
  • Spermatozoa
  • Trim27 hypomethylation

ASJC Scopus subject areas

  • Cell Biology
  • Reproductive Medicine

Fingerprint

Dive into the research topics of 'Nicotine induced murine spermatozoa apoptosis via up-regulation of deubiquitinated RIP1 by Trim27 promoter hypomethylation'. Together they form a unique fingerprint.

Cite this