Nitric Oxide Selectively Tunes Inhibitory Synapses to Modulate Vertebrate Locomotion

David L. McLean, Keith T. Sillar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

We have explored the possible modulation by nitric oxide (NO) of inhibitory synaptic transmission mediated by either glycine or GABA during episodes of rhythmic fictive swimming in postem-bryonic Xenopus laevis tadpoles. Extracellular ventral-root recordings suggest a stage-dependent increase in the reliability and extent of the NO donor S-nitroso-n-acetylpenicillamine (SNAP; 0.1-1 mM) to inhibit swimming by reducing the frequency and shortening the duration of swim episodes. These effects of SNAP on the swimming rhythm at both developmental stages are corroborated by intracellular recordings from presumed motor neurons with sharp microelectrodes, which also suggest that NO inhibits swimming by facilitating both glycinergic and GABAergic inhibition. However, we found no evidence for NO modulation of the excitatory drive for swimming. In addition to presynaptic effects on inhibitory transmitter release, a pronounced postsynaptic membrane depolarization (∼5-10 mV) and conductance decrease (∼10-20%) are associated with bath application of SNAR Hence, NO exerts inhibitory effects on swimming through multiple but selective actions on both the electrical properties of spinal neurons and on particular synaptic interconnections. The presynaptic and postsynaptic effects of NO act in concert to tune inhibitory synapses.

Original languageEnglish (US)
Pages (from-to)4175-4184
Number of pages10
JournalJournal of Neuroscience
Volume22
Issue number10
DOIs
StatePublished - May 15 2002

Keywords

  • GABA
  • Glycine
  • Locomotion
  • Nitric oxide
  • Release
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

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