Nitric oxide synthase type 3 is increased in squamous hyperplasia, dysplasia, and squamous cell carcinoma of the head and neck

Brandon G. Bentz, Harold J. Pelzer, G. Kenneth Haines, David G. Hanson, Mark W. Lingen, James A. Radosevich*

*Corresponding author for this work

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

The implication of nitric oxide (NO.) in the multistep process of carcinogenesis prompted us to examine the expression of endothelial constitutive nitric oxide synthase (NOS3) in head and neck squamous cell carcinoma (HNSCCa). Eleven paraffin-embedded samples of normal oral mucosa, 3 reactive oral lesions, 13 samples of squamous dysplasia, and 120 specimens of HNSCCa were immunostained with an anti-NOS3 monoclonal antibody and graded on a 0 to 4+ scale of intensity. Normal squamous mucosa demonstrated very little NOS3 expression. Areas of normal mucosa, reactive mucosa, and dysplastic lesions associated with inflammation tended to demonstrate regional expression of NOS3. Reactive mucosal lesions, squamous dysplasia, and HNSCCa demonstrated a significant (p < .0001) increase in global expression of NOS3. Therefore, NOS3 is expressed very little in histologically normal squamous mucosa, while squamous hyperplasia, dysplasia, and HNSCCa express significantly more NOS3. Regional variation in NOS3 expression appears to be associated with perilesional inflammation.

Original languageEnglish (US)
Pages (from-to)781-787
Number of pages7
JournalAnnals of Otology, Rhinology and Laryngology
Volume108
Issue number8
DOIs
StatePublished - Jan 1 1999

Keywords

  • Immunohistochemistry
  • Monoclonal antibody
  • Neoplasms
  • Nitric oxide
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Otorhinolaryngology

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