NMR characterization of the aliphatic β/β pairing for recognition of A·T/T·A base pairs in the minor groove of DNA

Rafael Peláez Lamamie De Clairac, Christian J. Seel, Bernhard H. Geierstanger, Milan Mrksich, Eldon E. Baird, Peter B. Dervan, David E. Wemmer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Polyamides containing N-methylimidazole (Im) and N-methylpyrrole (Py) amino acids can be combined in antiparallel side-by-side dimeric complexes for sequence-specific recognition in the minor groove of DNA. Because the curvature of four or five contiguous Im-Py rings does not perfectly match the canonical B-helix, β-alanine (β) residues have been inserted to reset the register. Complexes of three pyrrole-imidazole polyamides of sequence composition ImPyPy-X-PyPyPy-Dp, where X = Py, β, or glycine (G), bound to a 13 base pair DNA duplex containing a 9 base pair 5'-TGTATATCA-3' match site were characterized by NMR. NMR titrations and NOESY data combined with restrained molecular modeling show that each polyamide adopts an extended antiparallel dimeric conformation with the ligands fully overlapped around a central Py/Py, G/G, or β/β pair. Conformational exchange is seen near the linker for the G-linked complex, but not with the β or Py linkers. In addition to providing the first direct structural evidence for formation of the aliphatic β/β pairing in the minor groove, models support the idea that the β linker of ImPyPy-β-PyPyPy-Dp provides an optimal combination of size, flexibility, and alignment of the polyamide-paired aromatic subunits in extended, dimeric 2:1 complexes.

Original languageEnglish (US)
Pages (from-to)2956-2964
Number of pages9
JournalJournal of the American Chemical Society
Volume121
Issue number13
DOIs
StatePublished - Apr 7 1999

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'NMR characterization of the aliphatic β/β pairing for recognition of A·T/T·A base pairs in the minor groove of DNA'. Together they form a unique fingerprint.

Cite this