NMR Structural Studies on a Nonnatural Deoxyribonucleoside Which Mediates Recognition of GC Base Pairs in Pyrimidine·Purine·Pyrimidine DNA Triplexes

Ishwar Radhakrishnan, Dinshaw J. Patel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

As a part of our ongoing efforts to define the structural aspects of unusual pairing alignments in DNA triplexes by nuclear magnetic resonance spectroscopy, we have examined the structural role of a nonnatural deoxyribonucleoside, PI, that has been shown to mediate the recognition of GC base pairs in pyrimidine·purine·pyrimidine DNA triplexes [Koh, J. S., & Dervan, P. B. (1992) J. Am. Chem Soc. 114, 1470]. A qualitative interpretation of the NMR data indicates that this analog of protonated cytosine is readily accommodated in the third strand segment of an intramolecular triplex system. Furthermore, the observed NOE patterns position the imino and amino protons of PI opposite the N7 and O6 atoms of guanine, respectively, consistent with the previously proposed pairing scheme.

Original languageEnglish (US)
Pages (from-to)11228-11234
Number of pages7
JournalBiochemistry
Volume32
Issue number41
DOIs
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Biochemistry

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