Non-invasive diagnosis of early pulmonary disease in PECAM-deficient mice using infrared pulse oximetry

Merideth A. Early, Marta Lishnevsky, John M. Gilchrist, David M. Higgins, Ian M. Orme, William A. Muller, Mercedes Gonzalez-Juarerro, Alan R. Schenkel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Pulse oximetry is a common tool for detecting reduced pulmonary function in human interstitial lung diseases. It has not previously been used in a mouse model of interstitial lung disease. Further, platelet endothelial cell adhesion molecule deficient mice rarely show symptoms until disease is advanced. Using blood oxygen saturation, different stages of disease could be identified in a non-invasive manner. These stages could be correlated to pathology. Collagen deposition, using Picrosirius Red, did correlate with blood oxygen saturation. These studies are the first to show the use of an infrared pulse oximetry system to analyze the progression of a fibrotic interstitial lung disease in a mouse model of the human diseases. Further, these studies show that an early alveolar damage/enlargement event precedes the fibrosis in this mouse model, a stage that represents the best targets for disease analysis and prevention. This stage does not have extensive collagen deposition. Most importantly, targeting this earliest stage of disease for therapeutic intervention may lead to novel treatment for human disease.

Original languageEnglish (US)
Pages (from-to)152-158
Number of pages7
JournalExperimental and Molecular Pathology
Volume87
Issue number2
DOIs
StatePublished - Oct 2009

Keywords

  • Blood
  • Fibrosis
  • Lung
  • Oxygen
  • PECAM

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

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