Abstract
Cystic fibrosis transmembrane conductance regulator gene (CFTR) shows a complex mechanism of tissue-specific and temporal regulation. CFTR mRNA detection and measurement are extremely difficult because of the low to very low levels of its endogenous expression. In this paper, we describe four different non-PCR methods optimized to analyze CFTR transcripts in epithelial cell lines, primary cell lines and native tissues that express significant amounts of CFTR transcript.
Original language | English (US) |
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Pages (from-to) | 25-28 |
Number of pages | 4 |
Journal | Journal of Cystic Fibrosis |
Volume | 3 |
Issue number | SUPPL. 2 |
DOIs | |
State | Published - Aug 2004 |
Keywords
- In situ hybridization
- Northern blotting
- RACE
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Pediatrics, Perinatology, and Child Health