Non-PCR methods for the analysis of CFTR transcripts

Ann E O Trezise; Carlos M. Farinha; Ghanshyam D. Heda; Ann Harris; Margarida D. Amaral; Nathalie Mouchel

doi:10.1016/j.jcf.2004.05.005

Non-PCR methods for the analysis of CFTR transcripts

Ann E O Trezise, Carlos M. Farinha, Ghanshyam D. Heda, Ann Harris, Margarida D. Amaral, Nathalie Mouchel^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Cystic fibrosis transmembrane conductance regulator gene (CFTR) shows a complex mechanism of tissue-specific and temporal regulation. CFTR mRNA detection and measurement are extremely difficult because of the low to very low levels of its endogenous expression. In this paper, we describe four different non-PCR methods optimized to analyze CFTR transcripts in epithelial cell lines, primary cell lines and native tissues that express significant amounts of CFTR transcript.

Original language	English (US)
Pages (from-to)	25-28
Number of pages	4
Journal	Journal of Cystic Fibrosis
Volume	3
Issue number	SUPPL. 2
DOIs	https://doi.org/10.1016/j.jcf.2004.05.005
State	Published - Aug 2004

Keywords

In situ hybridization
Northern blotting
RACE

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Pulmonary and Respiratory Medicine

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10.1016/j.jcf.2004.05.005

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title = "Non-PCR methods for the analysis of CFTR transcripts",

abstract = "Cystic fibrosis transmembrane conductance regulator gene (CFTR) shows a complex mechanism of tissue-specific and temporal regulation. CFTR mRNA detection and measurement are extremely difficult because of the low to very low levels of its endogenous expression. In this paper, we describe four different non-PCR methods optimized to analyze CFTR transcripts in epithelial cell lines, primary cell lines and native tissues that express significant amounts of CFTR transcript.",

keywords = "In situ hybridization, Northern blotting, RACE",

author = "Trezise, {Ann E O} and Farinha, {Carlos M.} and Heda, {Ghanshyam D.} and Ann Harris and Amaral, {Margarida D.} and Nathalie Mouchel",

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AU - Trezise, Ann E O

AU - Farinha, Carlos M.

AU - Heda, Ghanshyam D.

AU - Harris, Ann

AU - Amaral, Margarida D.

AU - Mouchel, Nathalie

PY - 2004/8

Y1 - 2004/8

N2 - Cystic fibrosis transmembrane conductance regulator gene (CFTR) shows a complex mechanism of tissue-specific and temporal regulation. CFTR mRNA detection and measurement are extremely difficult because of the low to very low levels of its endogenous expression. In this paper, we describe four different non-PCR methods optimized to analyze CFTR transcripts in epithelial cell lines, primary cell lines and native tissues that express significant amounts of CFTR transcript.

AB - Cystic fibrosis transmembrane conductance regulator gene (CFTR) shows a complex mechanism of tissue-specific and temporal regulation. CFTR mRNA detection and measurement are extremely difficult because of the low to very low levels of its endogenous expression. In this paper, we describe four different non-PCR methods optimized to analyze CFTR transcripts in epithelial cell lines, primary cell lines and native tissues that express significant amounts of CFTR transcript.

KW - In situ hybridization

KW - Northern blotting

KW - RACE

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EP - 28

JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

IS - SUPPL. 2

ER -

Non-PCR methods for the analysis of CFTR transcripts

Abstract

Keywords

ASJC Scopus subject areas

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