Non-PCR methods for the analysis of CFTR transcripts

Ann E O Trezise, Carlos M. Farinha, Ghanshyam D. Heda, Ann Harris, Margarida D. Amaral, Nathalie Mouchel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Cystic fibrosis transmembrane conductance regulator gene (CFTR) shows a complex mechanism of tissue-specific and temporal regulation. CFTR mRNA detection and measurement are extremely difficult because of the low to very low levels of its endogenous expression. In this paper, we describe four different non-PCR methods optimized to analyze CFTR transcripts in epithelial cell lines, primary cell lines and native tissues that express significant amounts of CFTR transcript.

Original languageEnglish (US)
Pages (from-to)25-28
Number of pages4
JournalJournal of Cystic Fibrosis
Volume3
Issue numberSUPPL. 2
DOIs
StatePublished - Aug 2004

Keywords

  • In situ hybridization
  • Northern blotting
  • RACE

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Pulmonary and Respiratory Medicine

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