Nonclassical Ly6C- monocytes drive the development of inflammatory arthritis in mice

Alexander V. Misharin, Carla M. Cuda, Rana Saber, Jason D. Turner, Angelica K. Gierut, G. K. Kenneth Haines, Sergejs Berdnikovs, Andrew Filer, Andrew R. Clark, Christopher D. Buckley, Gökhan M. Mutlu, G. R. Scott Budinger, Harris Perlman*

*Corresponding author for this work

Research output: Contribution to journalArticle

127 Scopus citations

Abstract

Different subsets and/or polarized phenotypes of monocytes and macrophages may play distinct roles during the development and resolution of inflammation. Here, we demonstrate in a murine model of rheumatoid arthritis that nonclassical Ly6C- monocytes are required for the initiation and progression of sterile joint inflammation. Moreover, nonclassical Ly6C- monocytes differentiate into inflammatory macrophages (M1), which drive disease pathogenesis and display plasticity during the resolution phase. During the development of arthritis, these cells polarize toward an alternatively activated phenotype (M2), promoting the resolution of joint inflammation. The influx of Ly6C- monocytes and their subsequent classical and then alternative activation occurs without changes in synovial tissueresident macrophages, which express markers of M2 polarization throughout the course of the arthritis and attenuate joint inflammation during the initiation phase. These data suggest that circulating Ly6C- monocytes recruited to the joint upon injury orchestrate the development and resolution of autoimmune joint inflammation.

Original languageEnglish (US)
Pages (from-to)591-604
Number of pages14
JournalCell reports
Volume9
Issue number2
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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