Norepinephrine increases alveolar fluid reabsorption and Na,K-ATPase activity

Zaher S. Azzam, Yochai Adir, Astrid Crespo, Alejandro Comellas, Emilia Lecuona, Laura A. Dada, Norberto Krivoy, David H. Rutschman, Jacob I. Sznajder, Karen M. Ridge*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


The purpose of this study was to determine whether α-adrenergic receptor agonists have a role in alveolar fluid reabsorption, via Na,K-ATPase, in the alveolar epithelium. Alveolar fluid reabsorption increased approximately twofold with increasing concentrations of norepinephrine (NE) as compared with control rats. Treatment with the nonselective α-adrenergic receptor agonist, octopamine, and the specific a, agonist, phenylephrine, increased alveolar fluid reabsorption by 54 and 40%, respectively, as compared with control. The specific α1,-adrenergic receptor antagonist, prazosin, inhibited the stimulatory effects of NE by approximately 30%, whereas α2-adrenergic antagonist, yohimbine, did not prevent the stimulatory effects of NE. The administration of ouabain, Na,K-ATPase inhibitor, prevented the NE-mediated increase in alveolar fluid reabsorption. In parallel with these changes, NE increased Na,K-ATPase activity and protein abundance in alveolar epithelial type II cells via the α1- and β-adrenergic receptor. We report here that NE increased alveolar fluid reabsorption via the activation of both α1,- and β-adrenergic receptors, but not α2-adrenergic receptors. These effects are due to increased activity and abundance of the Na,K-ATPase in the basolateral membrane of ATII cells.

Original languageEnglish (US)
Pages (from-to)730-736
Number of pages7
JournalAmerican journal of respiratory and critical care medicine
Issue number7
StatePublished - Oct 1 2004


  • Active Na transport
  • Alveolar fluid reabsorption
  • Na,K-ATPase
  • Norepinephrine
  • α-adrenergic receptors

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine


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