Notch signaling induces either apoptosis or cell fate change in multiciliated cells during mucociliary tissue remodeling

Alexia Tasca, Martin Helmstädter, Magdalena Maria Brislinger, Maximilian Haas, Brian Mitchell, Peter Walentek*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Multiciliated cells (MCCs) are extremely highly differentiated, presenting >100 cilia and basal bodies. Therefore, MCC fate is thought to be terminal and irreversible. We analyzed how MCCs are removed from the airway-like mucociliary Xenopus epidermis during developmental tissue remodeling. We found that a subset of MCCs undergoes lateral line-induced apoptosis, but that the majority coordinately trans-differentiate into goblet secretory cells. Both processes are dependent on Notch signaling, while the cellular response to Notch is modulated by Jak/STAT, thyroid hormone, and mTOR signaling. At the cellular level, trans-differentiation is executed through the loss of ciliary gene expression, including foxj1 and pcm1, altered proteostasis, cilia retraction, basal body elimination, as well as the initiation of mucus production and secretion. Our work describes two modes for MCC loss during vertebrate development, the signaling regulation of these processes, and demonstrates that even cells with extreme differentiation features can undergo direct fate conversion.

Original languageEnglish (US)
JournalDevelopmental Cell
DOIs
StateAccepted/In press - 2021

Keywords

  • apoptosis
  • autophagy
  • basal body
  • cilia
  • goblet cell
  • mucus
  • pericentriolar material
  • proteasome
  • transdifferentiation
  • Xenopus

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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