Notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells

Chong Zhi Wang, Xiaoji Luo, Bin Zhang, Wen Xin Song, Ming Ni, Sangeeta Mehendale, Jing Tian Xie, Han H. Aung, Tong Chuan He, Chun Su Yuan*

*Corresponding author for this work

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Purpose: Panax notoginseng is a commonly used Chinese herb. Although a few studies have found that notoginseng shows anti-tumor effects, the effect of this herb on colorectal cancer cells has not been investigated. 5-Fluorouracil (5-FU) is a chemotherapeutic agent for the treatment of colorectal cancer that interferes with the growth of cancer cells. However, this compound has serious side effects at high doses. In this study, using HCT-116 human colorectal cancer cell line, we investigated the possible synergistic anti-cancer effects between notoginseng flower extract (NGF) and 5-FU on colon cancer cells. Methods: The anti-proliferation activity of these modes of treatment was evaluated by MTS cell proliferation assay. Apoptotic effects were analyzed by using Hoechst 33258 staining and Annexin-V/PI staining assays. The anti-proliferation effects of four major single compounds from NGF, ginsenosides Rb1, Rb3, Rc and Rg3 were also analyzed. Results: Both 5-FU and NGF inhibited proliferation of HCT-116 cells. With increasing doses of 5-FU, the anti-proliferation effect was slowly increased. The combined usage of 5-FU 5 μM and NGF 0.25 mg/ml, significantly increased the anti-proliferation effect (59.4 ± 3.3%) compared with using the two medicines separately (5-FU 5 μM, 31.1 ± 0.4%; NGF 0.25 mg/ml, 25.3 ± 3.6%). Apoptotic analysis showed that at this concentration, 5-FU did not exert an apoptotic effect, while apoptotic cells induced by NGF were observed, suggesting that the anti-proliferation target(s) of NGF may be different from that of 5-FU, which is known to inhibit thymidilate synthase. Conclusions: This study demonstrates that NGF can enhance the anti-proliferation effect of 5-FU on HCT-116 human colorectal cancer cells and may decrease the dosage of 5-FU needed for colorectal cancer treatment.

Original languageEnglish (US)
Pages (from-to)69-79
Number of pages11
JournalCancer Chemotherapy and Pharmacology
Volume60
Issue number1
DOIs
StatePublished - Jun 1 2007

Keywords

  • 5-Fluorouracil
  • Anti-proliferation
  • Apoptosis
  • Chemotherapy
  • HCT-116 human colorectal cancer cells
  • Panax notoginseng

ASJC Scopus subject areas

  • Oncology
  • Toxicology
  • Pharmacology
  • Cancer Research
  • Pharmacology (medical)

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  • Cite this

    Wang, C. Z., Luo, X., Zhang, B., Song, W. X., Ni, M., Mehendale, S., Xie, J. T., Aung, H. H., He, T. C., & Yuan, C. S. (2007). Notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells. Cancer Chemotherapy and Pharmacology, 60(1), 69-79. https://doi.org/10.1007/s00280-006-0350-2