Novel α4-integrin ligands on an endothelial cell line

Kim Sue R S Tudor, Tracy L. Deem, Joan M. Cook-Mills*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The unique combination of adhesion molecules expressed on endothelial cells is thought to mediate the specificity of leukocyte-endothelial cell interactions. In this study, murine endothelial cell lines were used as a model to identify novel adhesion molecules that participate in these cellular interactions. Lymphocyte adhesion to the continuous endothelial cell lines mHEVa and mHEVc required α4-integrin. Interestingly, lymphocyte α4-integrin bound to VCAM-1 as well as an unknown ligand on the mHEVa cell line. We have demonstrated that this VCAM-1-independent adhesion to the mHEVa cells was not mediated by other known α4-integrin ligands (fibronectin, α4-integrin itself, or MAdCAM-1). Two novel α4-integrin ligands (p50 and p10) were isolated from the mHEVa cell line but not the mHEVc cell line by B cell α4- integrin-specific ligand binding of radiolabeled mHEV cell membrane proteins. These results provide the first direct evidence that novel ligands for α4- integrin exist on membranes from endothelial cells.

Original languageEnglish (US)
Pages (from-to)99-113
Number of pages15
JournalBiochemistry and Cell Biology
Volume78
Issue number2
DOIs
StatePublished - 2000

Keywords

  • B cell
  • Endothelial cells
  • Integrin
  • T cell
  • VCAM-1
  • Vascular cell adhesion molecule-1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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