Abstract
Actin-crosslinking proteins link F-actin into the bundles and networks that constitute the cytoskeleton. Dystrophin, β-spectrin, α-actinin, ABP-120, ABP-280, and fimbrin share homologous actin-binding domains and comprise an actin crosslinker superfamily. We have identified a novel member of this superfamily (ACF7) using a degenerate primer-mediated PCR strategy that was optimized to resolve less-abundant superfamily sequences. The ACF7 gene is on human chromosome 1 and hybridizes to high molecular weight bands on northern blots. Sequence comparisons argue that ACF7 does not fit into one of the existing families, but represents a new class within the superfamily.
Original language | English (US) |
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Pages (from-to) | 500-504 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 368 |
Issue number | 3 |
DOIs | |
State | Published - Jul 24 1995 |
Funding
Acknowledgements: We thank Kiichi Arahata for isolating DNA from the Agtl0 library. This work was supported in part by National Institutes of Health Grant NS 23740 to LMK. AHB was supported in part by the Charles H. Hood Foundation. LMK is an investigator of the Howard Hughes Medical Institute.
Keywords
- Actin-binding protein
- Dystrophin
- Human brain
- Multigene family
- Polymerase chain reaction
- β-Spectrin
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Biophysics
- Structural Biology
- Biochemistry
- Cell Biology