Novel actin crosslinker superfamily member identified by a two step degenerate PCR procedure

Timothy J. Byers*, Alan H. Beggs, Elizabeth M. McNally, Louis M. Kunkel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Actin-crosslinking proteins link F-actin into the bundles and networks that constitute the cytoskeleton. Dystrophin, β-spectrin, α-actinin, ABP-120, ABP-280, and fimbrin share homologous actin-binding domains and comprise an actin crosslinker superfamily. We have identified a novel member of this superfamily (ACF7) using a degenerate primer-mediated PCR strategy that was optimized to resolve less-abundant superfamily sequences. The ACF7 gene is on human chromosome 1 and hybridizes to high molecular weight bands on northern blots. Sequence comparisons argue that ACF7 does not fit into one of the existing families, but represents a new class within the superfamily.

Original languageEnglish (US)
Pages (from-to)500-504
Number of pages5
JournalFEBS Letters
Volume368
Issue number3
DOIs
StatePublished - Jul 24 1995

Funding

Acknowledgements: We thank Kiichi Arahata for isolating DNA from the Agtl0 library. This work was supported in part by National Institutes of Health Grant NS 23740 to LMK. AHB was supported in part by the Charles H. Hood Foundation. LMK is an investigator of the Howard Hughes Medical Institute.

Keywords

  • Actin-binding protein
  • Dystrophin
  • Human brain
  • Multigene family
  • Polymerase chain reaction
  • β-Spectrin

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Cell Biology

Fingerprint

Dive into the research topics of 'Novel actin crosslinker superfamily member identified by a two step degenerate PCR procedure'. Together they form a unique fingerprint.

Cite this