Novel actin crosslinker superfamily member identified by a two step degenerate PCR procedure

Timothy J. Byers*, Alan H. Beggs, Elizabeth M. McNally, Louis M. Kunkel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Actin-crosslinking proteins link F-actin into the bundles and networks that constitute the cytoskeleton. Dystrophin, β-spectrin, α-actinin, ABP-120, ABP-280, and fimbrin share homologous actin-binding domains and comprise an actin crosslinker superfamily. We have identified a novel member of this superfamily (ACF7) using a degenerate primer-mediated PCR strategy that was optimized to resolve less-abundant superfamily sequences. The ACF7 gene is on human chromosome 1 and hybridizes to high molecular weight bands on northern blots. Sequence comparisons argue that ACF7 does not fit into one of the existing families, but represents a new class within the superfamily.

Original languageEnglish (US)
Pages (from-to)500-504
Number of pages5
JournalFEBS Letters
Volume368
Issue number3
DOIs
StatePublished - Jul 24 1995

Keywords

  • Actin-binding protein
  • Dystrophin
  • Human brain
  • Multigene family
  • Polymerase chain reaction
  • β-Spectrin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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