Novel actin crosslinker superfamily member identified by a two step degenerate PCR procedure

Timothy J. Byers; Alan H. Beggs; Elizabeth M. McNally; Louis M. Kunkel

doi:10.1016/0014-5793(95)00722-L

Novel actin crosslinker superfamily member identified by a two step degenerate PCR procedure

Timothy J. Byers^*, Alan H. Beggs, Elizabeth M. McNally, Louis M. Kunkel

^*Corresponding author for this work

Medicine, Cardiology Division

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Actin-crosslinking proteins link F-actin into the bundles and networks that constitute the cytoskeleton. Dystrophin, β-spectrin, α-actinin, ABP-120, ABP-280, and fimbrin share homologous actin-binding domains and comprise an actin crosslinker superfamily. We have identified a novel member of this superfamily (ACF7) using a degenerate primer-mediated PCR strategy that was optimized to resolve less-abundant superfamily sequences. The ACF7 gene is on human chromosome 1 and hybridizes to high molecular weight bands on northern blots. Sequence comparisons argue that ACF7 does not fit into one of the existing families, but represents a new class within the superfamily.

Original language	English (US)
Pages (from-to)	500-504
Number of pages	5
Journal	FEBS Letters
Volume	368
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)00722-L
State	Published - Jul 24 1995

Funding

Acknowledgements: We thank Kiichi Arahata for isolating DNA from the Agtl0 library. This work was supported in part by National Institutes of Health Grant NS 23740 to LMK. AHB was supported in part by the Charles H. Hood Foundation. LMK is an investigator of the Howard Hughes Medical Institute.

Keywords

Actin-binding protein
Dystrophin
Human brain
Multigene family
Polymerase chain reaction
β-Spectrin

ASJC Scopus subject areas

Genetics
Molecular Biology
Biophysics
Structural Biology
Biochemistry
Cell Biology

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10.1016/0014-5793(95)00722-L

Cite this

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title = "Novel actin crosslinker superfamily member identified by a two step degenerate PCR procedure",

abstract = "Actin-crosslinking proteins link F-actin into the bundles and networks that constitute the cytoskeleton. Dystrophin, β-spectrin, α-actinin, ABP-120, ABP-280, and fimbrin share homologous actin-binding domains and comprise an actin crosslinker superfamily. We have identified a novel member of this superfamily (ACF7) using a degenerate primer-mediated PCR strategy that was optimized to resolve less-abundant superfamily sequences. The ACF7 gene is on human chromosome 1 and hybridizes to high molecular weight bands on northern blots. Sequence comparisons argue that ACF7 does not fit into one of the existing families, but represents a new class within the superfamily.",

keywords = "Actin-binding protein, Dystrophin, Human brain, Multigene family, Polymerase chain reaction, β-Spectrin",

author = "Byers, \{Timothy J.\} and Beggs, \{Alan H.\} and McNally, \{Elizabeth M.\} and Kunkel, \{Louis M.\}",

note = "Funding Information: Acknowledgements: We thank Kiichi Arahata for isolating DNA from the Agtl0 library. This work was supported in part by National Institutes of Health Grant NS 23740 to LMK. AHB was supported in part by the Charles H. Hood Foundation. LMK is an investigator of the Howard Hughes Medical Institute.",

year = "1995",

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doi = "10.1016/0014-5793(95)00722-L",

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AU - Byers, Timothy J.

AU - Beggs, Alan H.

AU - McNally, Elizabeth M.

AU - Kunkel, Louis M.

N1 - Funding Information: Acknowledgements: We thank Kiichi Arahata for isolating DNA from the Agtl0 library. This work was supported in part by National Institutes of Health Grant NS 23740 to LMK. AHB was supported in part by the Charles H. Hood Foundation. LMK is an investigator of the Howard Hughes Medical Institute.

PY - 1995/7/24

Y1 - 1995/7/24

N2 - Actin-crosslinking proteins link F-actin into the bundles and networks that constitute the cytoskeleton. Dystrophin, β-spectrin, α-actinin, ABP-120, ABP-280, and fimbrin share homologous actin-binding domains and comprise an actin crosslinker superfamily. We have identified a novel member of this superfamily (ACF7) using a degenerate primer-mediated PCR strategy that was optimized to resolve less-abundant superfamily sequences. The ACF7 gene is on human chromosome 1 and hybridizes to high molecular weight bands on northern blots. Sequence comparisons argue that ACF7 does not fit into one of the existing families, but represents a new class within the superfamily.

AB - Actin-crosslinking proteins link F-actin into the bundles and networks that constitute the cytoskeleton. Dystrophin, β-spectrin, α-actinin, ABP-120, ABP-280, and fimbrin share homologous actin-binding domains and comprise an actin crosslinker superfamily. We have identified a novel member of this superfamily (ACF7) using a degenerate primer-mediated PCR strategy that was optimized to resolve less-abundant superfamily sequences. The ACF7 gene is on human chromosome 1 and hybridizes to high molecular weight bands on northern blots. Sequence comparisons argue that ACF7 does not fit into one of the existing families, but represents a new class within the superfamily.

KW - Actin-binding protein

KW - Dystrophin

KW - Human brain

KW - Multigene family

KW - Polymerase chain reaction

KW - β-Spectrin

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ER -

Novel actin crosslinker superfamily member identified by a two step degenerate PCR procedure

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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