Abstract
Actin-crosslinking proteins link F-actin into the bundles and networks that constitute the cytoskeleton. Dystrophin, β-spectrin, α-actinin, ABP-120, ABP-280, and fimbrin share homologous actin-binding domains and comprise an actin crosslinker superfamily. We have identified a novel member of this superfamily (ACF7) using a degenerate primer-mediated PCR strategy that was optimized to resolve less-abundant superfamily sequences. The ACF7 gene is on human chromosome 1 and hybridizes to high molecular weight bands on northern blots. Sequence comparisons argue that ACF7 does not fit into one of the existing families, but represents a new class within the superfamily.
Original language | English (US) |
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Pages (from-to) | 500-504 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 368 |
Issue number | 3 |
DOIs | |
State | Published - Jul 24 1995 |
Keywords
- Actin-binding protein
- Dystrophin
- Human brain
- Multigene family
- Polymerase chain reaction
- β-Spectrin
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology