TY - JOUR
T1 - Novel nanoscale delivery particles encapsulated with anticancer drugs, all-trans retinoic acid or curcumin, enhance apoptosis in lymphoma cells predominantly expressing CD20 antigen
AU - Stauffer, Richard G.
AU - Mohammad, Manar
AU - Singh, Amareshwar T K
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Background: Mantle cell lymphoma (MCL), a B-cell lymphoma, pursues a relatively aggressive course, is resistant to long-term remission, and is associated with a poor prognosis. There is a pressing need for innovative treatment approaches against MCL. One such approach is targeted delivery of cytotoxic drugs to MCL cells. Materials and Methods: In the current investigation, we pursued a strategy to employ retinoid-based or curcumin-based nanoscale delivery particles, called nanodisks (NDs), for targeted drug delivery to MCL cells (Granta), and human follicular lymphoma (HF-1) cells. The cells were incubated with NDs made of CD20 single-chain variable antibody fragment (scFv)/apolipoprotein A-1 fusion protein, and loaded with either all-trans retinoic acid (ATRA) or curcumin, and cell apoptosis was measured using flow cytometry. Results and Conclusion: At 10 μM, curcumin-ND induced cell death more effectively than ATRA-ND. Combination of curcumin-ND and ATRA-ND significantly enhanced the biological activity of these drugs against lymphoma cells compared to individual treatments.
AB - Background: Mantle cell lymphoma (MCL), a B-cell lymphoma, pursues a relatively aggressive course, is resistant to long-term remission, and is associated with a poor prognosis. There is a pressing need for innovative treatment approaches against MCL. One such approach is targeted delivery of cytotoxic drugs to MCL cells. Materials and Methods: In the current investigation, we pursued a strategy to employ retinoid-based or curcumin-based nanoscale delivery particles, called nanodisks (NDs), for targeted drug delivery to MCL cells (Granta), and human follicular lymphoma (HF-1) cells. The cells were incubated with NDs made of CD20 single-chain variable antibody fragment (scFv)/apolipoprotein A-1 fusion protein, and loaded with either all-trans retinoic acid (ATRA) or curcumin, and cell apoptosis was measured using flow cytometry. Results and Conclusion: At 10 μM, curcumin-ND induced cell death more effectively than ATRA-ND. Combination of curcumin-ND and ATRA-ND significantly enhanced the biological activity of these drugs against lymphoma cells compared to individual treatments.
KW - All-trans retinoic acid
KW - Apoptosis
KW - Curcumin
KW - Lymphoma
KW - Nanodisks
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M3 - Article
C2 - 26637852
AN - SCOPUS:84949439809
VL - 35
SP - 6425
EP - 6430
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 12
ER -