Abstract
Three types of polycationic phospholipids conjugates were synthesized to develop a novel nonviral gene carrier. The conjugates composed of a polyamine portion: spermidine, spermine or polyethylenimine (PEI), and a phospholipid portion: dipalmitoylphosphatidic acid (DPPA) were synthesized via a synthetic intermediate, ethylbrominated DPPA. The polycationic lipids exhibited moderate transfection efficacy evaluated by β-galactosidase assay. These conjugates form a micelle-like assembly in aqueous solution, whose sizes are in the range of 90∼220 nm. The formation of the conjugate-DNA complex was observed by using atomic force microscopy (AFM). The AFM images revealed that the conjugate-bearing spermidine, which showed the most efficient transfection, enables one to form a compact complex (∼130 nm in width) with DNA, while the conjugate-bearing PEI, which showed a less efficient transfection, forms a large complex (∼200 nm) with DNA.
Original language | English (US) |
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Pages (from-to) | 634-639 |
Number of pages | 6 |
Journal | Kobunshi Ronbunshu |
Volume | 61 |
Issue number | 12 |
DOIs | |
State | Published - Dec 2004 |
Keywords
- AFM
- Compaction
- Gene transfer
- Polyamine
- Polycationic lipid
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Materials Science (miscellaneous)
- General Environmental Science
- Polymers and Plastics