Novel Stable DNA Nanoscale Material and Its Application on Specific Enrichment of DNA

Xueting Wang, Jia Yu, Wenjie Lan, Shuo Yang, Shiqing Wang, Yue Mi, Qing Ye, Yuan Li, Yin Liu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

DNA nanostructures are a new type of technology for constructing nanomaterials that has been developed in recent years. By relying on the complementary pairing of DNA molecules to form a double-stranded property, DNA molecules can construct a variety of nanoscale structures of 2D and 3D shapes. However, most of the previously reported DNA nanostructures rely solely on hydrogen bonds to maintain structural stability, resulting in DNA structures that can be maintained only at low temperature and in the presence of Mg2+, which greatly limits the application of DNA nanostructures. This study designed a DNA nanonetwork structure (nanonet) and changed its topological structure to DNA nanomesh by using DNA topoisomerase to make it thermally stable, while escaping the dependence on Mg2+, and the stability of the structure can be maintained in a nonsolution state. Moreover, the nanomesh also has a large amount of ssDNA (about 50%), providing active sites capable of exerting biological functions. Using the above characteristics, we prepared the nanomesh into a device capable of adsorbing specific DNA molecules, and used the device to enrich DNA. We also tried to mount antibodies using DNA probes. Preliminary results show that the DNA nanomesh also has the ability to enrich specific proteins.

Original languageEnglish (US)
Pages (from-to)19834-19839
Number of pages6
JournalACS Applied Materials and Interfaces
Volume12
Issue number17
DOIs
StatePublished - Apr 29 2020

Keywords

  • DNA self-assembly
  • DNA topoisomerase
  • nanomesh
  • nanostructure
  • specific enrichment

ASJC Scopus subject areas

  • Materials Science(all)

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