TY - JOUR
T1 - NSG mice provide a better spontaneous model of breast cancer metastasis than athymic (nude) mice
AU - Puchalapalli, Madhavi
AU - Zeng, Xianke
AU - Mu, Liang
AU - Anderson, Aubree
AU - Glickman, Laura Hix
AU - Zhang, Ming
AU - Sayyad, Megan R.
AU - Wangensteen, Sierra Mosticone
AU - Clevenger, Charles V.
AU - Koblinski, Jennifer E.
N1 - Funding Information:
The authors would like to thank Yvonne Feeney, Briana Ratchford, and Candice Jones for technical help with tissue processing. This work was financially supported by funding from the Lynn Sage Cancer Research Foundation and the Zell Foundation, Chicago, IL. Imaging work was performed at the Northwestern University Center for Advanced Microscopy generously supported by NCI CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center. Services and products in support of the research project were generated by the VCU Massey Cancer Center Biological Macromolecule Shared Resource, supported, in part, with funding from NIH-NCI Cancer Center Support Grant P30 CA016059.
Publisher Copyright:
© 2016 Puchalapalli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/9
Y1 - 2016/9
N2 - Metastasis is the most common cause of mortality in breast cancer patients worldwide. To identify improved mouse models for breast cancer growth and spontaneous metastasis, we examined growth and metastasis of both estrogen receptor positive (T47D) and negative (MDA-MB-231, SUM1315, and CN34BrM) human breast cancer cells in nude and NSG mice. Both primary tumor growth and spontaneous metastases were increased in NSG mice compared to nude mice. In addition, a pattern of metastasis similar to that observed in human breast cancer patients (metastases to the lungs, liver, bones, brain, and lymph nodes) was found in NSG mice. Furthermore, there was an increase in the metastatic burden in NSG compared to nude mice that were injected with MDA-MB-231 breast cancer cells in an intracardiac experimental metastasis model. This data demonstrates that NSG mice provide a better model for studying human breast cancer metastasis compared to the current nude mouse model.
AB - Metastasis is the most common cause of mortality in breast cancer patients worldwide. To identify improved mouse models for breast cancer growth and spontaneous metastasis, we examined growth and metastasis of both estrogen receptor positive (T47D) and negative (MDA-MB-231, SUM1315, and CN34BrM) human breast cancer cells in nude and NSG mice. Both primary tumor growth and spontaneous metastases were increased in NSG mice compared to nude mice. In addition, a pattern of metastasis similar to that observed in human breast cancer patients (metastases to the lungs, liver, bones, brain, and lymph nodes) was found in NSG mice. Furthermore, there was an increase in the metastatic burden in NSG compared to nude mice that were injected with MDA-MB-231 breast cancer cells in an intracardiac experimental metastasis model. This data demonstrates that NSG mice provide a better model for studying human breast cancer metastasis compared to the current nude mouse model.
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U2 - 10.1371/journal.pone.0163521
DO - 10.1371/journal.pone.0163521
M3 - Article
C2 - 27662655
AN - SCOPUS:84992079902
SN - 1932-6203
VL - 11
JO - PLoS One
JF - PLoS One
IS - 9
M1 - e0163521
ER -
