Nuclear egress of pseudorabies virus capsids is enhanced by a subspecies of the large tegument protein that is lost upon cytoplasmic maturation

Mindy Leelawong, Joy I. Lee, Gregory A. Smith*

*Corresponding author for this work

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Herpesviruses morphogenesis occurs stepwise both temporally and spatially, beginning in the nucleus and concluding with the emergence of an extracellular virion. The mechanisms by which these viruses interact with and penetrate the nuclear envelope and subsequent compartments of the secretory pathway remain poorly defined. In this report, a conserved viral protein (VP1/2; pUL36) that directs cytoplasmic stages of egress is identified to have multiple isoforms. Of these, a novel truncated VP1/2 species translocates to the nucleus and assists the transfer of DNA-containing capsids to the cytoplasm. The capsids are handed off to full-length VP1/2, which replaces the nuclear isoform on the capsids and is required for the final cytoplasmic stages of viral particle maturation. These results document that distinct VP1/2 protein species serve as effectors of nuclear and cytoplasmic egress.

Original languageEnglish (US)
Pages (from-to)6303-6314
Number of pages12
JournalJournal of virology
Volume86
Issue number11
DOIs
StatePublished - Jun 1 2012

ASJC Scopus subject areas

  • Immunology
  • Virology

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