Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis

Barbara Boettcher*, Tatiana T. Marquez-Lago, Mathias Bayer, Eric L. Weiss, Yves Barral

*Corresponding author for this work

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

During vegetative growth, Saccharomyces cerevisiae cells divide asymmetrically: the mother cell buds to produce a smaller daughter cell. This daughter asymmetrically inherits the transcription factor Ace2, which activates daughter-specific transcriptional programs. In this paper, we investigate when and how this asymmetry is established and maintained. We show that Ace2 asymmetry is initiated in the elongated, but undivided, anaphase nucleus. At this stage, the nucleoplasm was highly compartmentalized; little exchange was observed for nucleoplasmic proteins between mother and bud. Using photobleaching and in silico modeling, we show that diffusion barriers compartmentalize the nuclear membranes. In contrast, the behavior of proteins in the nucleoplasm is well explained by the dumbbell shape of the anaphase nucleus. This compartmentalization of the nucleoplasm promoted Ace2 asymmetry in anaphase nuclei. Thus, our data indicate that yeast cells use the process of closed mitosis and the morphological constraints associated with it to asymmetrically segregate nucleoplasmic components.

Original languageEnglish (US)
Pages (from-to)921-937
Number of pages17
JournalJournal of Cell Biology
Volume197
Issue number7
DOIs
StatePublished - Jun 25 2012

ASJC Scopus subject areas

  • Cell Biology

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