Nuclear import of serum response factor in airway smooth muscle

John F. McConville; Darren J. Fernandes; Jason Churchill; Samantha Dewundara; Paul Kogut; Shardul Shah; Gregory Fuchs; Dalius Kedainis; Shashi K. Bellam; Nina M. Patel; Joel McCauley; Nickolai O. Dulin; Mahesh P. Gupta; Stephen Adam; Yoshihiro Yoneda; Blanca Camoretti-Mercado; Julian Solway

doi:10.1165/rcmb.2008-0393OC

Nuclear import of serum response factor in airway smooth muscle

John F. McConville, Darren J. Fernandes, Jason Churchill, Samantha Dewundara, Paul Kogut, Shardul Shah, Gregory Fuchs, Dalius Kedainis, Shashi K. Bellam, Nina M. Patel, Joel McCauley, Nickolai O. Dulin, Mahesh P. Gupta, Stephen Adam, Yoshihiro Yoneda, Blanca Camoretti-Mercado, Julian Solway^*

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We have previously shown that the transcription-promoting activity of serum response factor (SRF) is partially regulated by its extranuclear redistribution. In this study, we examined the cellular mechanisms that facilitate SRFnuclear entry in canine tracheal smooth muscle cells. We used in vitro pull-down assays to determine which karyopherin proteins bound SRF and found that SRF binds KPNA1 and KPNB1 through its nuclear localization sequence. Immunoprecipitation studies also demonstrated direct SRF-KPNA1 interaction in HEK293 cells. Import assays demonstrated that KPNA1 and KPNB1 together were sufficient tomediate rapid nuclear import of SRF-GFP. Our studies also suggest that SRF is able to gain nuclear entry through an auxiliary, nuclear localization sequence-independent mechanism.

Original language	English (US)
Pages (from-to)	453-458
Number of pages	6
Journal	American journal of respiratory cell and molecular biology
Volume	45
Issue number	3
DOIs	https://doi.org/10.1165/rcmb.2008-0393OC
State	Published - Sep 1 2011

Keywords

Karyopherin
Nuclear import
Serum response factor

ASJC Scopus subject areas

Molecular Biology
Pulmonary and Respiratory Medicine
Clinical Biochemistry
Cell Biology

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10.1165/rcmb.2008-0393OC

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McConville, J. F., Fernandes, D. J., Churchill, J., Dewundara, S., Kogut, P., Shah, S., Fuchs, G., Kedainis, D., Bellam, S. K., Patel, N. M., McCauley, J., Dulin, N. O., Gupta, M. P., Adam, S., Yoneda, Y., Camoretti-Mercado, B., & Solway, J. (2011). Nuclear import of serum response factor in airway smooth muscle. American journal of respiratory cell and molecular biology, 45(3), 453-458. https://doi.org/10.1165/rcmb.2008-0393OC

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title = "Nuclear import of serum response factor in airway smooth muscle",

abstract = "We have previously shown that the transcription-promoting activity of serum response factor (SRF) is partially regulated by its extranuclear redistribution. In this study, we examined the cellular mechanisms that facilitate SRFnuclear entry in canine tracheal smooth muscle cells. We used in vitro pull-down assays to determine which karyopherin proteins bound SRF and found that SRF binds KPNA1 and KPNB1 through its nuclear localization sequence. Immunoprecipitation studies also demonstrated direct SRF-KPNA1 interaction in HEK293 cells. Import assays demonstrated that KPNA1 and KPNB1 together were sufficient tomediate rapid nuclear import of SRF-GFP. Our studies also suggest that SRF is able to gain nuclear entry through an auxiliary, nuclear localization sequence-independent mechanism.",

keywords = "Karyopherin, Nuclear import, Serum response factor",

author = "McConville, {John F.} and Fernandes, {Darren J.} and Jason Churchill and Samantha Dewundara and Paul Kogut and Shardul Shah and Gregory Fuchs and Dalius Kedainis and Bellam, {Shashi K.} and Patel, {Nina M.} and Joel McCauley and Dulin, {Nickolai O.} and Gupta, {Mahesh P.} and Stephen Adam and Yoshihiro Yoneda and Blanca Camoretti-Mercado and Julian Solway",

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doi = "10.1165/rcmb.2008-0393OC",

language = "English (US)",

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journal = "American journal of respiratory cell and molecular biology",

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McConville, JF, Fernandes, DJ, Churchill, J, Dewundara, S, Kogut, P, Shah, S, Fuchs, G, Kedainis, D, Bellam, SK, Patel, NM, McCauley, J, Dulin, NO, Gupta, MP, Adam, S, Yoneda, Y, Camoretti-Mercado, B & Solway, J 2011, 'Nuclear import of serum response factor in airway smooth muscle', American journal of respiratory cell and molecular biology, vol. 45, no. 3, pp. 453-458. https://doi.org/10.1165/rcmb.2008-0393OC

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AU - McConville, John F.

AU - Fernandes, Darren J.

AU - Churchill, Jason

AU - Dewundara, Samantha

AU - Kogut, Paul

AU - Shah, Shardul

AU - Fuchs, Gregory

AU - Kedainis, Dalius

AU - Bellam, Shashi K.

AU - Patel, Nina M.

AU - McCauley, Joel

AU - Dulin, Nickolai O.

AU - Gupta, Mahesh P.

AU - Adam, Stephen

AU - Yoneda, Yoshihiro

AU - Camoretti-Mercado, Blanca

AU - Solway, Julian

PY - 2011/9/1

Y1 - 2011/9/1

N2 - We have previously shown that the transcription-promoting activity of serum response factor (SRF) is partially regulated by its extranuclear redistribution. In this study, we examined the cellular mechanisms that facilitate SRFnuclear entry in canine tracheal smooth muscle cells. We used in vitro pull-down assays to determine which karyopherin proteins bound SRF and found that SRF binds KPNA1 and KPNB1 through its nuclear localization sequence. Immunoprecipitation studies also demonstrated direct SRF-KPNA1 interaction in HEK293 cells. Import assays demonstrated that KPNA1 and KPNB1 together were sufficient tomediate rapid nuclear import of SRF-GFP. Our studies also suggest that SRF is able to gain nuclear entry through an auxiliary, nuclear localization sequence-independent mechanism.

AB - We have previously shown that the transcription-promoting activity of serum response factor (SRF) is partially regulated by its extranuclear redistribution. In this study, we examined the cellular mechanisms that facilitate SRFnuclear entry in canine tracheal smooth muscle cells. We used in vitro pull-down assays to determine which karyopherin proteins bound SRF and found that SRF binds KPNA1 and KPNB1 through its nuclear localization sequence. Immunoprecipitation studies also demonstrated direct SRF-KPNA1 interaction in HEK293 cells. Import assays demonstrated that KPNA1 and KPNB1 together were sufficient tomediate rapid nuclear import of SRF-GFP. Our studies also suggest that SRF is able to gain nuclear entry through an auxiliary, nuclear localization sequence-independent mechanism.

KW - Karyopherin

KW - Nuclear import

KW - Serum response factor

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JO - American journal of respiratory cell and molecular biology

JF - American journal of respiratory cell and molecular biology

IS - 3

ER -

Nuclear import of serum response factor in airway smooth muscle

Abstract

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