Nuclear import of serum response factor in airway smooth muscle

John F. McConville, Darren J. Fernandes, Jason Churchill, Samantha Dewundara, Paul Kogut, Shardul Shah, Gregory Fuchs, Dalius Kedainis, Shashi K. Bellam, Nina M. Patel, Joel McCauley, Nickolai O. Dulin, Mahesh P. Gupta, Stephen Adam, Yoshihiro Yoneda, Blanca Camoretti-Mercado, Julian Solway*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


We have previously shown that the transcription-promoting activity of serum response factor (SRF) is partially regulated by its extranuclear redistribution. In this study, we examined the cellular mechanisms that facilitate SRFnuclear entry in canine tracheal smooth muscle cells. We used in vitro pull-down assays to determine which karyopherin proteins bound SRF and found that SRF binds KPNA1 and KPNB1 through its nuclear localization sequence. Immunoprecipitation studies also demonstrated direct SRF-KPNA1 interaction in HEK293 cells. Import assays demonstrated that KPNA1 and KPNB1 together were sufficient tomediate rapid nuclear import of SRF-GFP. Our studies also suggest that SRF is able to gain nuclear entry through an auxiliary, nuclear localization sequence-independent mechanism.

Original languageEnglish (US)
Pages (from-to)453-458
Number of pages6
JournalAmerican journal of respiratory cell and molecular biology
Issue number3
StatePublished - Sep 1 2011


  • Karyopherin
  • Nuclear import
  • Serum response factor

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology


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