Nuclear magnetic resonance structural studies of A · AT base triple alignments in intramolecular purine · purine · pyrimidine DNA triplexes in solution

Ishwar Radhakrishnan, Carlos De Los Santos, Dinshaw J. Patel

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

This study reports on an NMR characterization of an intramolecular purine · purine · pyrimidine (R · RY) triplex containing a, central A · AT triple in addition to G · GC and T · AT triples. Our studies establish an A(anti) · AT alignment with reversed Hoogsteen pairing stabilized by two N6H . . . N hydrogen bonds between adenine residues in the third strand and the purine strand of the duplex. This result, combined with our earlier demonstration of G(anti) · GC and T(anti) · AT pairing alignments, has provided a definitive experimental approach for differentiating between the base triple pairing possibilities proposed previously by Beal and Dervan for R · RY triplexes.

Original languageEnglish (US)
Pages (from-to)188-197
Number of pages10
JournalJournal of Molecular Biology
Volume234
Issue number1
DOIs
StatePublished - Nov 5 1993

Keywords

  • A · AT triples
  • NMR
  • R · RY triplexes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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