Nuclease-resistant DNA via high-density packing in polymeric micellar nanoparticle coronas

Anthony M. Rush, Matthew P. Thompson, Erick T. Tatro, Nathan C. Gianneschi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Herein, we describe a polymeric micellar nanoparticle capable of rendering nucleic acids resistant to nuclease digestion. This approach relies on utilizing DNA as the polar headgroup of a DNA-polymer amphiphile in order to assemble well-defined, discrete nanoparticles. Dense packing of DNA in the micelle corona allows for hybridization of complementary oligonucleotides while prohibiting enzymatic degradation. We demonstrate the preparation, purification, and characterization of the nanoparticles, then describe their resistance to treatment with endo- and exonucleases including snake-venom phosphodiesterase (SVP), a common, general DNA digestion enzyme.

Original languageEnglish (US)
Pages (from-to)1379-1387
Number of pages9
JournalACS nano
Volume7
Issue number2
DOIs
StatePublished - Feb 26 2013

Keywords

  • DNA
  • nanotechnology
  • nuclease
  • polymer
  • resistance

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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