Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes

Benoit Palancade*, Xianpeng Liu, Maria Garcia-Rubio, Andrès Aguilera, Xianlan Zhao, Valérip Doye

*Corresponding author for this work

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Increasing evidences suggest that nuclear pore complexes (NPCs) control different aspects of nuclear metabolism, including transcription, nuclear organization, and DNA repair. We previously established that the Nup84 complex, a major NPC building block, is part of a genetic network involved in DNA repair. Here, we show that double-strand break (DSB) appearance is linked to a shared function of the Nup84 and the Nup60/Mlp1-2 complexes. Mutants within these complexes exhibit similar genetic interactions and alteration in DNA repair processes as mutants of the SUMO-protease Ulp1. Consistently, these nucleoporins are required for maintenance of proper Ulp1 levels at NPCs and for the establishment of the appropriate sumoylation of several cellular proteins, including the DNA repair factor Yku70. Moreover, restoration of nuclear envelope-associated Ulp1 in nucleoporin mutants reestablishes proper sumoylation patterns and suppresses DSB accumulation and genetic interactions with DNA repair genes. Our results thus provide a molecular mechanism that underlies the connection between NPC and genome stability.

Original languageEnglish (US)
Pages (from-to)2912-2923
Number of pages12
JournalMolecular biology of the cell
Volume18
Issue number8
DOIs
StatePublished - Aug 1 2007

Fingerprint

Nuclear Pore Complex Proteins
Sumoylation
Nuclear Pore
DNA Repair
DNA Damage
Genomic Instability
Nuclear Envelope
Maintenance
Genes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Palancade, Benoit ; Liu, Xianpeng ; Garcia-Rubio, Maria ; Aguilera, Andrès ; Zhao, Xianlan ; Doye, Valérip. / Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes. In: Molecular biology of the cell. 2007 ; Vol. 18, No. 8. pp. 2912-2923.
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Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes. / Palancade, Benoit; Liu, Xianpeng; Garcia-Rubio, Maria; Aguilera, Andrès; Zhao, Xianlan; Doye, Valérip.

In: Molecular biology of the cell, Vol. 18, No. 8, 01.08.2007, p. 2912-2923.

Research output: Contribution to journalArticle

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