Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase

J. R. Sampson; A. B. DiRenzo; L. S. Behlen; O. C. Uhlenbeck

doi:10.1126/science.2646717

Nucleotides in yeast tRNA^Phe required for the specific recognition by its cognate synthetase

J. R. Sampson, A. B. DiRenzo, L. S. Behlen, O. C. Uhlenbeck

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

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Abstract

An analysis of the aminoacylation kinetics of unmodified yeast tRNA ^Phe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.

Original language	English (US)
Pages (from-to)	1363-1366
Number of pages	4
Journal	Science
Volume	243
Issue number	4896
DOIs	https://doi.org/10.1126/science.2646717
State	Published - 1989

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title = "Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase",

abstract = "An analysis of the aminoacylation kinetics of unmodified yeast tRNA Phe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.",

author = "Sampson, {J. R.} and DiRenzo, {A. B.} and Behlen, {L. S.} and Uhlenbeck, {O. C.}",

year = "1989",

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volume = "243",

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AU - DiRenzo, A. B.

AU - Behlen, L. S.

AU - Uhlenbeck, O. C.

PY - 1989

Y1 - 1989

N2 - An analysis of the aminoacylation kinetics of unmodified yeast tRNA Phe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.

AB - An analysis of the aminoacylation kinetics of unmodified yeast tRNA Phe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.

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Nucleotides in yeast tRNA^Phe required for the specific recognition by its cognate synthetase

Abstract

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