Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase

J. R. Sampson, A. B. DiRenzo, L. S. Behlen, O. C. Uhlenbeck

Research output: Contribution to journalArticlepeer-review

145 Scopus citations

Abstract

An analysis of the aminoacylation kinetics of unmodified yeast tRNA Phe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.

Original languageEnglish (US)
Pages (from-to)1363-1366
Number of pages4
JournalScience
Volume243
Issue number4896
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • General

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