Nutritional requirements of human induced pluripotent stem cells

Davi M. Lyra-Leite; Raymond R. Copley; Phillip P. Freeman; Praeploy Pongpamorn; Disheet Shah; Donald E. McKenna; Brian Lenny; Emily A. Pinheiro; Carly J. Weddle; Mennat Gharib; Hoor Javed; Hananeh Fonoudi; Yadav Sapkota; Paul W. Burridge

doi:10.1016/j.stemcr.2023.05.004

Nutritional requirements of human induced pluripotent stem cells

Davi M. Lyra-Leite, Raymond R. Copley, Phillip P. Freeman, Praeploy Pongpamorn, Disheet Shah, Donald E. McKenna, Brian Lenny, Emily A. Pinheiro, Carly J. Weddle, Mennat Gharib, Hoor Javed, Hananeh Fonoudi, Yadav Sapkota, Paul W. Burridge^*

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The nutritional requirements for human induced pluripotent stem cell (hiPSC) growth have not been extensively studied. Here, building on our prior work that established the suitable non-basal medium components for hiPSC growth, we develop a simplified basal medium consisting of just 39 components, demonstrating that many ingredients of DMEM/F12 are either not essential or are at suboptimal concentrations. This new basal medium along with the supplement, which we call BMEM, enhances the growth rate of hiPSCs over DMEM/F12-based media, supports derivation of multiple hiPSC lines, and allows differentiation to multiple lineages. hiPSCs cultured in BMEM consistently have enhanced expression of undifferentiated cell markers such as POU5F1 and NANOG, along with increased expression of markers of the primed state and reduced expression of markers of the naive state. This work describes titration of the nutritional requirements of human pluripotent cell culture and identifies that suitable nutrition enhances the pluripotent state.

Original language	English (US)
Pages (from-to)	1371-1387
Number of pages	17
Journal	Stem cell reports
Volume	18
Issue number	6
DOIs	https://doi.org/10.1016/j.stemcr.2023.05.004
State	Published - Jun 13 2023

Funding

This work was supported by NIH R01 grants CA220002 and CA261898, the Leducq Foundation (to P.W.B.), and the American Heart Association Postdoctoral Fellowship 874276 (D.M.L.-L.). Metabolomics services were performed by the Metabolomics Core Facility at Robert H. Lurie Comprehensive Cancer Center of Northwestern University. R.R.C. P.P.F. and D.E.M. are employees of Clever Carnivore, Inc. P.W.B is a co-founder of Clever Carnivore, Inc. This work was supported by NIH R01 grants CA220002 and CA261898 , the Leducq Foundation (to P.W.B.), and the American Heart Association Postdoctoral Fellowship 874276 (D.M.L.-L.). Metabolomics services were performed by the Metabolomics Core Facility at Robert H. Lurie Comprehensive Cancer Center of Northwestern University.

Keywords

Cell culture media
chemically defined
human induced pluripotent stem cell

ASJC Scopus subject areas

Genetics
Biochemistry
Cell Biology
Developmental Biology

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10.1016/j.stemcr.2023.05.004

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title = "Nutritional requirements of human induced pluripotent stem cells",

abstract = "The nutritional requirements for human induced pluripotent stem cell (hiPSC) growth have not been extensively studied. Here, building on our prior work that established the suitable non-basal medium components for hiPSC growth, we develop a simplified basal medium consisting of just 39 components, demonstrating that many ingredients of DMEM/F12 are either not essential or are at suboptimal concentrations. This new basal medium along with the supplement, which we call BMEM, enhances the growth rate of hiPSCs over DMEM/F12-based media, supports derivation of multiple hiPSC lines, and allows differentiation to multiple lineages. hiPSCs cultured in BMEM consistently have enhanced expression of undifferentiated cell markers such as POU5F1 and NANOG, along with increased expression of markers of the primed state and reduced expression of markers of the naive state. This work describes titration of the nutritional requirements of human pluripotent cell culture and identifies that suitable nutrition enhances the pluripotent state.",

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note = "Funding Information: This work was supported by NIH R01 grants CA220002 and CA261898, the Leducq Foundation (to P.W.B.), and the American Heart Association Postdoctoral Fellowship 874276 (D.M.L.-L.). Metabolomics services were performed by the Metabolomics Core Facility at Robert H. Lurie Comprehensive Cancer Center of Northwestern University. R.R.C. P.P.F. and D.E.M. are employees of Clever Carnivore, Inc. P.W.B is a co-founder of Clever Carnivore, Inc. Funding Information: This work was supported by NIH R01 grants CA220002 and CA261898 , the Leducq Foundation (to P.W.B.), and the American Heart Association Postdoctoral Fellowship 874276 (D.M.L.-L.). Metabolomics services were performed by the Metabolomics Core Facility at Robert H. Lurie Comprehensive Cancer Center of Northwestern University. Publisher Copyright: {\textcopyright} 2023 The Author(s)",

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month = jun,

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doi = "10.1016/j.stemcr.2023.05.004",

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AU - Lyra-Leite, Davi M.

AU - Copley, Raymond R.

AU - Freeman, Phillip P.

AU - Pongpamorn, Praeploy

AU - Shah, Disheet

AU - McKenna, Donald E.

AU - Lenny, Brian

AU - Pinheiro, Emily A.

AU - Weddle, Carly J.

AU - Gharib, Mennat

AU - Javed, Hoor

AU - Fonoudi, Hananeh

AU - Sapkota, Yadav

AU - Burridge, Paul W.

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PY - 2023/6/13

Y1 - 2023/6/13

N2 - The nutritional requirements for human induced pluripotent stem cell (hiPSC) growth have not been extensively studied. Here, building on our prior work that established the suitable non-basal medium components for hiPSC growth, we develop a simplified basal medium consisting of just 39 components, demonstrating that many ingredients of DMEM/F12 are either not essential or are at suboptimal concentrations. This new basal medium along with the supplement, which we call BMEM, enhances the growth rate of hiPSCs over DMEM/F12-based media, supports derivation of multiple hiPSC lines, and allows differentiation to multiple lineages. hiPSCs cultured in BMEM consistently have enhanced expression of undifferentiated cell markers such as POU5F1 and NANOG, along with increased expression of markers of the primed state and reduced expression of markers of the naive state. This work describes titration of the nutritional requirements of human pluripotent cell culture and identifies that suitable nutrition enhances the pluripotent state.

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Nutritional requirements of human induced pluripotent stem cells

Abstract

Funding

Keywords

ASJC Scopus subject areas

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