Odorants as cell-type specific activators of a heat shock response in the rat olfactory mucosa

Virginian Mc Millan Carr, Bert Ph M. Menco, Maya P. Yankova, Richard I. Morimoto, Albert I. Farbman

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Heat shock, or stress, proteins (HSPs) are induced in response to conditions that cause protein denaturation. Activation of cellular stress responses as a protective and survival mechanism is often associated with chemical exposure. One interface between the body and the external environment and chemical or biological agents therein is the olfactory epithelium (OE). To determine whether environmental odorants affect OE HSP expression, rats were exposed to a variety of odorants added to the cage bedding. Odorant exposure led to transient, selective induction of HSP70, HSC70, HSP25, and ubiquitin immunoreactivities (IRs) in supporting cells and subepithelial Bowman's gland acinar cells, two OE non-neuronal cell populations involved with inhalant biotransformation, detoxification, and maintenance of overall OE integrity. Responses exhibited odor specificity and dose dependency. HSP70 and HSC70 IRs occurred throughout the apical region of supporting cells; ubiquitin IR was confined to a supranuclear cone-shaped region. Electron microscopic examination confirmed these observations and, additionally, revealed odor-induced formation of dense vesicular arrays in the cone-like regions. HSP25 IR occurred throughout the entire supporting cell cytoplasm. In contrast to classical stress responses, in which the entire array of stress proteins is induced, no increases in HSP40 and HSP90 IRs were observed. Extended exposure to higher odorant doses caused prolonged activation of the same HSP subset in the nonneuronal cells and severe morphological damage in both supporting cells and olfactory receptor neurons (ORNs), suggesting that non-neuronal cytoprotective stress response mechanisms had been overwhelmed and could no longer adequately maintain OE integrity. Significantly, ORNs showed no stress responses in any of our studies. These findings suggest a novel role for these HSPs in olfaction and, in turn, possible involvement in other normal neurophysiological processes.

Original languageEnglish (US)
Pages (from-to)425-439
Number of pages15
JournalJournal of Comparative Neurology
Volume432
Issue number4
DOIs
StatePublished - Apr 16 2001

Keywords

  • Bowman's glands
  • HSC70
  • HSP25
  • HSP70
  • Olfactory epithelium
  • Olfactory supporting cells
  • Ubiquitin
  • Ubiquitin-positive membranous arrays

ASJC Scopus subject areas

  • Neuroscience(all)

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