Oligonucleotide-modified gold nanoparticles for infracellular gene regulation

Nathaniel L. Rosi, David A. Giljohann, C. Shad Thaxton, Abigail K R Lytton-Jean, Min Su Han, Chad A. Mirkin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1609 Scopus citations

Abstract

We describe the use of gold nanopartide-oligonucleotide complexes as intracellular gene regulation agents for the control of protein expression in cells. These oligonucleotide-modified nanoparticles have affinity constants for complementary nucleic acids that are higher than their unmodified oligonucleotide counterparts, are less susceptible to degradation by nuclease activity, exhibit greater than 99% cellular uptake, can introduce oligonucleotides at a higher effective concentration than conventional transfection agents, and are nontoxic to the cells under the conditions studied. By chemically tailoring the density of DNA bound to the surface of gold nanoparticles, we demonstrated a tunable gene knockdown.

Original languageEnglish (US)
Pages (from-to)1027-1030
Number of pages4
JournalScience
Volume312
Issue number5776
DOIs
StatePublished - May 19 2006

ASJC Scopus subject areas

  • General

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