@article{029df0767b784dc689cfd2052857575d,
title = "On-demand biomanufacturing of protective conjugate vaccines",
abstract = "Conjugate vaccines are among the most effective methods for preventing bacterial infections. However, existing manufacturing approaches limit access to conjugate vaccines due to centralized production and cold chain distribution requirements. To address these limitations, we developed a modular technology for in vitro conjugate vaccine expression (iVAX) in portable, freeze-dried lysates from detoxified, nonpathogenic Escherichia coli. Upon rehydration, iVAX reactions synthesize clinically relevant doses of conjugate vaccines against diverse bacterial pathogens in 1 hour. We show that iVAX-synthesized vaccines against Francisella tularensis subsp. tularensis (type A) strain Schu S4 protected mice from lethal intranasal F. tularensis challenge. The iVAX platform promises to accelerate development of new conjugate vaccines with increased access through refrigeration-independent distribution and portable production.",
author = "Stark, {Jessica C.} and Thapakorn Jaroentomeechai and Moeller, {Tyler D.} and Hershewe, {Jasmine M.} and Warfel, {Katherine F.} and Moricz, {Bridget S.} and Martini, {Anthony M.} and Dubner, {Rachel S.} and Hsu, {Karen J.} and Stevenson, {Taylor C.} and Jones, {Bradley D.} and DeLisa, {Matthew P.} and Jewett, {Michael C.}",
note = "Funding Information: This work was supported by Defense Threat Reduction Agency (HDTRA1-15-10052/P00001 and HDTRA1-20-1-0004 to M.P.D. and M.C.J.), National Science Foundation (grant nos. CBET 1159581 and CBET 1264701 both to M.P.D. and MCB 1413563 to M.P.D. and M.C.J.), the Bill and Melinda Gates Foundation (OPP1217652 to M.P.D. and M.C.J.), the David and Lucile Packard Foundation, and the Dreyfus Teacher-Scholar program. J.C.S. and T.C.S. were supported by National Science Foundation Graduate Research Fellowships. T.J. was supported by a Royal Thai Government Fellowship. T.D.M. was supported by a National Institutes of Health T32 Training Grant Fellowship and a Cornell Fleming Graduate Scholarship. J.M.H. and K.F.W. were supported by National Defense Science and Engineering Graduate Fellowships. R.S.D. was funded, in part, by the Northwestern University Chemistry of Life Processes Summer Scholars program. Publisher Copyright: Copyright {\textcopyright} 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).",
year = "2021",
month = feb,
day = "3",
doi = "10.1126/sciadv.abe9444",
language = "English (US)",
volume = "7",
journal = "Science advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "6",
}
