Oncogene expression in T-cell lymphoproliferative disorders

Gayle E. Woloschak*, W. Craig Hooper, Mary J. Doerge, Robert L. Phyliky, Thomas E. Witzig, Peter M. Banks, Gordon W. Dewald, Chin Yang Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We have investigated the expression of oncogenes and other related genes in eleven patients with T-cell lymphoproliferative disorders and ten patients with other hematologic malignancies. The phenotypes of the T-cell disorders were determined using monoclonal antibodies specific for helper or suppressor subsets. RNA preparations were isolated from peripheral blood mononuclear cells and/ or lymph node sections, 5′-end labeled with γ-32P-ATP, and hybridized under stringent conditions to an excess of nitrocellulose-bound specific cloned DNA; autoradiographs were analysed by microdensitometry. Results revealed increased expression of K-ras, v-fps, transferrin receptor, α-tubulin and α-interferon in at least five of six helper T-cell lymphoproliferative disorders, while five of five suppressor T-cell disorders demonstrated levels of hybridization to these clones no higher than background. However, studies of T-suppressor disorders demonstrated enhanced levels of β-interferon-specific RNA in five of five patients, an increase apparent in three of six T-helper chronic lymphoproliferative disorders. These results demonstrate different patterns of gene expression evident in T-helper and T-suppressor abnormalities.

Original languageEnglish (US)
Pages (from-to)327-337
Number of pages11
JournalLeukemia Research
Volume12
Issue number4
DOIs
StatePublished - 1988

Funding

Acknowledgements--We gratefully acknowledge the support of American Cancer Society Grant No.IM-348, Fraternal Order of Eagles Grant No. 50, a gift from Mrs Edythe Warner and the Mayo Foundation. The authors wish to thank Kathy Jensen for typing this manuscript and Drs Rebecca S. Bahn, Greg Wiseman, and John Lust for helpful suggestions after reviewing the manuscript. This work was presented in part at the 1986 FASEB meetings in St Louis, MO.

Keywords

  • Oncogenes
  • T-cells
  • gene expression
  • interferon
  • leukemia
  • lymphoma
  • lymphoproliferation

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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