Online capillary LC-FTMS for the analysis of covalent enzyme intermediates of polyketide and nonribosomal peptide synthetases

Matthew T. Mazur*, Leah M. Miller, Shaun M. McLoughlin, Lusong Luo, Leslle M. Hicks, Jun Wang, Christopher T. Walsh, Neil L. Kelleher

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The covalent enzymes intermediates of polyketide and nonribosomal peptide synthetases (NRPS) were analyzed. The study was carried out by using online capillary liquid chromatography Fourier transform mass spectroscopy (LC-FTMS). The enzymes involved in NRPS were often the largest in their respective proteosome and analysis requires enzymetic and chemical digestions followed by fractionations with reverse-phase chromatography. The results show that the rising complexity of subsequent digestion mixtures, chromatographic separation has had to be employed to achieve biosynthetic intermediates.

Original languageEnglish (US)
Title of host publicationProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages357-358
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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