Online capillary LC-FTMS for the analysis of covalent enzyme intermediates of polyketide and nonribosomal peptide synthetases

Matthew T. Mazur; Leah M. Miller; Shaun M. McLoughlin; Lusong Luo; Leslle M. Hicks; Jun Wang; Christopher T. Walsh; Neil L. Kelleher

Online capillary LC-FTMS for the analysis of covalent enzyme intermediates of polyketide and nonribosomal peptide synthetases

Matthew T. Mazur^*, Leah M. Miller, Shaun M. McLoughlin, Lusong Luo, Leslle M. Hicks, Jun Wang, Christopher T. Walsh, Neil L. Kelleher

^*Corresponding author for this work

Molecular Biosciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The covalent enzymes intermediates of polyketide and nonribosomal peptide synthetases (NRPS) were analyzed. The study was carried out by using online capillary liquid chromatography Fourier transform mass spectroscopy (LC-FTMS). The enzymes involved in NRPS were often the largest in their respective proteosome and analysis requires enzymetic and chemical digestions followed by fractionations with reverse-phase chromatography. The results show that the rising complexity of subsequent digestion mixtures, chromatographic separation has had to be employed to achieve biosynthetic intermediates.

Original language	English (US)
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	357-358
Number of pages	2
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

@inproceedings{fbab94d81b5f40cab91ed4d2ea1cfde7,

title = "Online capillary LC-FTMS for the analysis of covalent enzyme intermediates of polyketide and nonribosomal peptide synthetases",

abstract = "The covalent enzymes intermediates of polyketide and nonribosomal peptide synthetases (NRPS) were analyzed. The study was carried out by using online capillary liquid chromatography Fourier transform mass spectroscopy (LC-FTMS). The enzymes involved in NRPS were often the largest in their respective proteosome and analysis requires enzymetic and chemical digestions followed by fractionations with reverse-phase chromatography. The results show that the rising complexity of subsequent digestion mixtures, chromatographic separation has had to be employed to achieve biosynthetic intermediates.",

author = "Mazur, {Matthew T.} and Miller, {Leah M.} and McLoughlin, {Shaun M.} and Lusong Luo and Hicks, {Leslle M.} and Jun Wang and Walsh, {Christopher T.} and Kelleher, {Neil L.}",

year = "2002",

month = dec,

day = "1",

language = "English (US)",

pages = "357--358",

booktitle = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Mazur, MT, Miller, LM, McLoughlin, SM, Luo, L, Hicks, LM, Wang, J, Walsh, CT & Kelleher, NL 2002, Online capillary LC-FTMS for the analysis of covalent enzyme intermediates of polyketide and nonribosomal peptide synthetases. in Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 357-358, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.

Online capillary LC-FTMS for the analysis of covalent enzyme intermediates of polyketide and nonribosomal peptide synthetases. / Mazur, Matthew T.; Miller, Leah M.; McLoughlin, Shaun M. et al.

Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 357-358.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Online capillary LC-FTMS for the analysis of covalent enzyme intermediates of polyketide and nonribosomal peptide synthetases

AU - Mazur, Matthew T.

AU - Miller, Leah M.

AU - McLoughlin, Shaun M.

AU - Luo, Lusong

AU - Hicks, Leslle M.

AU - Wang, Jun

AU - Walsh, Christopher T.

AU - Kelleher, Neil L.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The covalent enzymes intermediates of polyketide and nonribosomal peptide synthetases (NRPS) were analyzed. The study was carried out by using online capillary liquid chromatography Fourier transform mass spectroscopy (LC-FTMS). The enzymes involved in NRPS were often the largest in their respective proteosome and analysis requires enzymetic and chemical digestions followed by fractionations with reverse-phase chromatography. The results show that the rising complexity of subsequent digestion mixtures, chromatographic separation has had to be employed to achieve biosynthetic intermediates.

AB - The covalent enzymes intermediates of polyketide and nonribosomal peptide synthetases (NRPS) were analyzed. The study was carried out by using online capillary liquid chromatography Fourier transform mass spectroscopy (LC-FTMS). The enzymes involved in NRPS were often the largest in their respective proteosome and analysis requires enzymetic and chemical digestions followed by fractionations with reverse-phase chromatography. The results show that the rising complexity of subsequent digestion mixtures, chromatographic separation has had to be employed to achieve biosynthetic intermediates.

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M3 - Conference contribution

AN - SCOPUS:2442683150

SP - 357

EP - 358

BT - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Online capillary LC-FTMS for the analysis of covalent enzyme intermediates of polyketide and nonribosomal peptide synthetases

Abstract

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ASJC Scopus subject areas

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