Open-Source Multiparametric Optocardiography

Brianna Cathey; Sofian Obaid; Alexander M. Zolotarev; Roman A. Pryamonosov; Roman A. Syunyaev; Sharon A. George; Igor R. Efimov

doi:10.1038/s41598-018-36809-y

Open-Source Multiparametric Optocardiography

Brianna Cathey, Sofian Obaid, Alexander M. Zolotarev, Roman A. Pryamonosov, Roman A. Syunyaev, Sharon A. George^*, Igor R. Efimov

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Since the 1970s fluorescence imaging has become a leading tool in the discovery of mechanisms of cardiac function and arrhythmias. Gradual improvements in fluorescent probes and multi-camera technology have increased the power of optical mapping and made a major impact on the field of cardiac electrophysiology. Tandem-lens optical mapping systems facilitated simultaneous recording of multiple parameters characterizing cardiac function. However, high cost and technological complexity restricted its proliferation to the wider biological community. We present here, an open-source solution for multiple-camera tandem-lens optical systems for multiparametric mapping of transmembrane potential, intracellular calcium dynamics and other parameters in intact mouse hearts and in rat heart slices. This 3D-printable hardware and Matlab-based RHYTHM 1.2 analysis software are distributed under an MIT open-source license. Rapid prototyping permits the development of inexpensive, customized systems with broad functionality, allowing wider application of this technology outside biomedical engineering laboratories.

Original language	English (US)
Article number	721
Journal	Scientific reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-36809-y
State	Published - Dec 1 2019

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10.1038/s41598-018-36809-y

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title = "Open-Source Multiparametric Optocardiography",

abstract = "Since the 1970s fluorescence imaging has become a leading tool in the discovery of mechanisms of cardiac function and arrhythmias. Gradual improvements in fluorescent probes and multi-camera technology have increased the power of optical mapping and made a major impact on the field of cardiac electrophysiology. Tandem-lens optical mapping systems facilitated simultaneous recording of multiple parameters characterizing cardiac function. However, high cost and technological complexity restricted its proliferation to the wider biological community. We present here, an open-source solution for multiple-camera tandem-lens optical systems for multiparametric mapping of transmembrane potential, intracellular calcium dynamics and other parameters in intact mouse hearts and in rat heart slices. This 3D-printable hardware and Matlab-based RHYTHM 1.2 analysis software are distributed under an MIT open-source license. Rapid prototyping permits the development of inexpensive, customized systems with broad functionality, allowing wider application of this technology outside biomedical engineering laboratories.",

author = "Brianna Cathey and Sofian Obaid and Zolotarev, {Alexander M.} and Pryamonosov, {Roman A.} and Syunyaev, {Roman A.} and George, {Sharon A.} and Efimov, {Igor R.}",

note = "Funding Information: We gratefully acknowledge support by a grant from the Foundation Leducq and NIH grants R21 EB023106, R01 HL126802, and R01 HL114395. We also gratefully acknowledge Russian Foundation for Basic Research grant 18-00-01524. R.S. research was supported by Russian Science Foundation grant 18-71-10058. We would like to thank the following people who helped conduct this study: Mark Wagner, Jaclyn Brennan, and Zexu Lin. Mark Wagner provided access and assistance in utilizing the university{\textquoteright}s 3D-printers. Jaclyn Brennan performed the experiment to collect ECG data. Zexu Lin provided cardiac rat slices used in system demonstration. Publisher Copyright: {\textcopyright} 2019, The Author(s).",

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doi = "10.1038/s41598-018-36809-y",

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AU - Cathey, Brianna

AU - Obaid, Sofian

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AU - Pryamonosov, Roman A.

AU - Syunyaev, Roman A.

AU - George, Sharon A.

AU - Efimov, Igor R.

N1 - Funding Information: We gratefully acknowledge support by a grant from the Foundation Leducq and NIH grants R21 EB023106, R01 HL126802, and R01 HL114395. We also gratefully acknowledge Russian Foundation for Basic Research grant 18-00-01524. R.S. research was supported by Russian Science Foundation grant 18-71-10058. We would like to thank the following people who helped conduct this study: Mark Wagner, Jaclyn Brennan, and Zexu Lin. Mark Wagner provided access and assistance in utilizing the university’s 3D-printers. Jaclyn Brennan performed the experiment to collect ECG data. Zexu Lin provided cardiac rat slices used in system demonstration. Publisher Copyright: © 2019, The Author(s).

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N2 - Since the 1970s fluorescence imaging has become a leading tool in the discovery of mechanisms of cardiac function and arrhythmias. Gradual improvements in fluorescent probes and multi-camera technology have increased the power of optical mapping and made a major impact on the field of cardiac electrophysiology. Tandem-lens optical mapping systems facilitated simultaneous recording of multiple parameters characterizing cardiac function. However, high cost and technological complexity restricted its proliferation to the wider biological community. We present here, an open-source solution for multiple-camera tandem-lens optical systems for multiparametric mapping of transmembrane potential, intracellular calcium dynamics and other parameters in intact mouse hearts and in rat heart slices. This 3D-printable hardware and Matlab-based RHYTHM 1.2 analysis software are distributed under an MIT open-source license. Rapid prototyping permits the development of inexpensive, customized systems with broad functionality, allowing wider application of this technology outside biomedical engineering laboratories.

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Open-Source Multiparametric Optocardiography

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