Opposite-polarity motors activate one another to trigger cargo transport in live cells

Shabeen Ally, Adam G. Larson, Kari Barlan, Sarah E. Rice, Vladimir I. Gelfand

Research output: Contribution to journalArticlepeer-review

158 Scopus citations

Abstract

Intracellular transport is typically bidirectional, consisting of a series of back and forth movements. Kinesin-1 and cytoplasmic dynein require each other for bidirectional transport of intracellular cargo along microtubules; i.e., inhibition or depletion of kinesin-1 abolishes dyneindriven cargo transport and vice versa. Using Drosophila melanogaster S2 cells, we demonstrate that replacement of endogenous kinesin-1 or dynein with an unrelated, peroxisome-targeted motor of the same directionality activates peroxisome transport in the opposite direction. However, motility-deficient versions of motors, which retain the ability to bind microtubules and hydrolyze adenosine triphosphate, do not activate peroxisome motility. Thus, any pair of opposite-polarity motors, provided they move along microtubules, can activate one another. These results demonstrate that mechanical interactions between opposite-polarity motors are necessary and sufficient for bidirectional organelle transport in live cells.

Original languageEnglish (US)
Pages (from-to)1071-1082
Number of pages12
JournalJournal of Cell Biology
Volume187
Issue number7
DOIs
StatePublished - Dec 28 2009

ASJC Scopus subject areas

  • Cell Biology

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