Abstract
We developed optical coherence photoacoustic microscopy (OC-PAM) to demonstrate that the functions of optical coherence tomography (OCT) and photoacoustic microscopy (PAM) can be achieved simultaneously by using a single illuminating light source. We used a pulsed broadband laser centered at 580nm and detected the absorbed photons through photoacoustic detection and the back-scattered photons with an interferometer. In OC-PAM, each laser pulse generates both one OCT A-line and one PAM A-line simultaneously; as a result, the two imaging modalities are intrinsically co-registered in the lateral directions. In vivo images of the mouse ear were acquired to demonstrate the capabilities of OC-PAM.
| Original language | English (US) |
|---|---|
| Article number | 030502 |
| Journal | Journal of Biomedical Optics |
| Volume | 17 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 2012 |
Funding
This work is supported in part by the following grants: National Institutes of Health grant 7R21EB008800-02 and Coulter Translational Award to S. Jiao; National Institutes of Health grant 1R01EY019951-01A1 to S. Jiao and HF Zhang; National Institutes of Health grant 1RC4EY021357 and National Science Foundation CBET-1055379 to HF Zhang.
Keywords
- hemoglobin
- melanin
- optical coherence tomography
- photoacoustic image
- photoacoustic microscopy
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering
- Biomaterials